Anti-RanBP2 抗体 (ab64276)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RanBP2 antibody
RanBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RanBP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:
SKAPKSGFEGMFTKKE
, amino acids 1592-1607. -
ポジティブ･コントロール
- WB: Wild-type HeLa, HeLa and Caco2 whole cell lysates. ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64276の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
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| ICC/IF | (3) |
1/2000.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa). |
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ICC/IF
1/2000. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
パスウェイ
Protein modification; protein sumoylation. -
関連疾患
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
配列類似性
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
ドメイン
Contains F-X-F-G repeats. -
翻訳後修飾
Polyubiquitinated by PARK2, which leads to proteasomal degradation. -
細胞内局在
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
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別名
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
画像
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Western blot - Anti-RanBP2 antibody (ab64276)All lanes : Anti-RanBP2 antibody (ab64276) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RANBP2 knockout HeLa cell lysate
Lane 3 : Caco2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 358 kDa
Observed band size: 450 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab64276 observed at 450 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab64276 was shown to react with RanBP2 in wild-type HeLa cells in Western blot with loss of signal observed in RANBP2 knockout cell line ab265618 (RANBP2 knockout cell lysate ab257627). Wild-type HeLa and RANBP2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab64276 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1:1000 dilution and 1:20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1:20000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate
Predicted band size: 358 kDa
Observed band size: 358 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy. -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000). -
Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate
Predicted band size: 358 kDa
Exposure time: 1 minute
WB performed on a 6%SDS-PAGE gel.Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab64276 は 33 報の論文で使用されています。
- Yi J et al. Role of Nesprin-2 and RanBP2 in BICD2-associated brain developmental disorders. PLoS Genet 19:e1010642 (2023). PubMed: 36930595
- Xue G et al. The HIV-1 capsid core is an opportunistic nuclear import receptor. Nat Commun 14:3782 (2023). PubMed: 37355754
- Betancor G et al. MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation. mBio 13:e0171422 (2022). PubMed: 35880880
- Guedán A et al. HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and integration. PLoS Pathog 17:e1009484 (2021). PubMed: 34543344
- Betancor G et al. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat Microbiol 6:1031-1042 (2021). PubMed: 34282309