Anti-RanBP2 抗体 (ab2938)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RanBP2 antibody
RanBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RanBP2 -
由来種
Rabbit -
特異性
Detects Ran binding protein 2 (RanBP 2) from human and bovine retina. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Cow, Human
交差が予測される動物種: Chimpanzee
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免疫原
Synthetic peptide corresponding to Human RanBP2 aa 1-18.
Sequence:MRRSKADVERYIASVQGS
(Peptide available asab4939) -
ポジティブ･コントロール
- IHC-P: Bovine retinal extracts. WB: Human retinal extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2938の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells. |
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939).
Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939). Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts. |
ターゲット情報
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機能
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
パスウェイ
Protein modification; protein sumoylation. -
関連疾患
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
配列類似性
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
ドメイン
Contains F-X-F-G repeats. -
翻訳後修飾
Polyubiquitinated by PARK2, which leads to proteasomal degradation. -
細胞内局在
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 735654 Chimpanzee
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: H2QII6 Chimpanzee
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- Unigene: 199561 Human
see all -
別名
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (ab2938)
Immunohistochemical staining of RanBP2 in bovine retina using ab2938.
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Western blot - Anti-RanBP2 antibody (ab2938)Western blot of RanBP2 from human retinal extracts using ab2938.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab2938 は 9 報の論文で使用されています。
- Li H et al. Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/ß-catenin signaling. J Exp Clin Cancer Res 38:33 (2019). PubMed: 30678687
- Saito A et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol 90:5808-23 (2016). PubMed: 27076642
- Henning MS et al. In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868 (2014). WB ; Human . PubMed: 24415937
- Ocwieja KE et al. HIV integration targeting: a pathway involving Transportin-3 and the nuclear pore protein RanBP2. PLoS Pathog 7:e1001313 (2011). WB ; Human . PubMed: 21423673
- Nagai M et al. Mice lacking Ran binding protein 1 are viable and show male infertility. FEBS Lett 585:791-6 (2011). IHC ; Mouse . PubMed: 21310149
- Zhang R et al. Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA). PLoS One 5:e15620 (2010). WB ; Human . PubMed: 21179483
- Felix RS et al. SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis. Cancer Lett 278:41-8 (2009). IHC-P ; Human . PubMed: 19171422
- Shitashige M et al. Regulation of Wnt signaling by the nuclear pore complex. Gastroenterology 134:1961-71, 1971.e1-4 (2008). PubMed: 18439914
- Jain P et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). Dot blot . PubMed: 17897946