JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB97659

Anti-RanBP1 抗体

Anti-RanBP1 antibody

Be the first to review this product! Submit a review

|

(11 Publications)

Rabbit Polyclonal RanBP1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RANBP1 aa 1-200.

別名を表示する

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB97659)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB97659)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Ran BP1 protein at cytoplasm using ab97659 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-RanBP1 antibody (AB97659)
  • WB

Supplier Data

Western blot - Anti-RanBP1 antibody (AB97659)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 24 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human RANBP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P43487

style
left-column

代替製品

Primary Antibodies

AB133550

Anti-RanBP1 antibody [EPR6796(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

  • 0
  • 0
Primary Antibodies

AB217940

Anti-RanBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (AB217940)

  • 0
  • 0
Primary Antibodies

AB2937

Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB2937)

  • 0
  • 0
Primary Antibodies

AB264129

Anti-RanBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (AB264129)

  • 0
  • 0
Primary Antibodies

AB70185

Anti-RanBP1 antibody

Immunoprecipitation - Anti-RanBP1 antibody (AB70185)

  • 0
  • 0
Primary Antibodies

AB17034

Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB191875

Anti-DGCR8 antibody [EPR18757]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DGCR8 antibody [EPR18757] (AB191875)

  • 5
  • 2
Primary Antibodies

AB316336

Anti-RanBP2 antibody [RM2047]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody [RM2047] (AB316336)

  • 0
  • 0
Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.
Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).
See full target information RANBP1
style
left-column

文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6548 PubMed40670348

2025

Elaboration of molecular glues that target TRIM21 into TRIMTACs that degrade protein aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Marc A Scemama de Gialluly,Anthony R Allen,Elijah H Hayes,Patrick Zhuang,Ralston B Goldfarb,Amanda N Farrar,Yuriy Fedorov,Drew J Adams

eLife 13: PubMed40420562

2025

An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Pratibha Thakur,Martin Lackinger,Anastasia Diamantopoulou,Sneha Rao,Yijing Chen,Khakima Khalizova,Annie Ferng,Curt Mazur,Holly Kordasiewicz,Robert J Shprintzen,Sander Markx,Bin Xu,Joseph A Gogos

Cell chemical biology 31:1363-1372.e8 PubMed38917791

2024

C646 degrades Exportin-1 to modulate p300 chromatin occupancy and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Fan Chen,Atikur Rahman,Joel L Sax,Matthew J Atala Pleshinger,Ryan M Friedrich,Drew J Adams

Human molecular genetics 32:1959-1974 PubMed36790128

2023

Ranbp1 modulates morphogenesis of the craniofacial midline in mouse models of 22q11.2 deletion syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Paronett,Corey A Bryan,Megan E Maynard,Julia A Goroff,Daniel W Meechan,Anthony-Samuel LaMantia,Thomas M Maynard

Pharmaceuticals (Basel, Switzerland) 14: PubMed34832913

2021

Inhibition of XPO-1 Mediated Nuclear Export through the Michael-Acceptor Character of Chalcones.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Gargantilla,José López-Fernández,Maria-Jose Camarasa,Leentje Persoons,Dirk Daelemans,Eva-Maria Priego,María-Jesús Pérez-Pérez

Cell reports 24:2529-2539.e4 PubMed30184488

2018

RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Mencarelli,Justyna Nitarska,Tim Kroecher,Francesco Ferraro,Katherine Massey,Antonella Riccio,Franck Pichaud

Cellular signalling 46:76-82 PubMed29501729

2018

STAT6 is a cargo of exportin 1: Biological relevance in primary mediastinal B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hadjer Miloudi,Karen Leroy,Fabrice Jardin,Brigitte Sola

Cell death & disease 7:e2497 PubMed27906185

2016

Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia.

Applications

WB

Species

Human

Vibeke Andresen,Bjarte S Erikstein,Herschel Mukherjee,André Sulen,Mihaela Popa,Steinar Sørnes,Håkon Reikvam,Kok-Ping Chan,Randi Hovland,Emmet McCormack,Øystein Bruserud,Andrew G Myers,Bjørn T Gjertsen

Oncotarget 7:68842-68850 PubMed27634897

2016

Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jasper Edgar Neggers,Els Vanstreels,Erkan Baloglu,Sharon Shacham,Yosef Landesman,Dirk Daelemans

Chemistry & biology 22:107-16 PubMed25579209

2015

Identifying drug-target selectivity of small-molecule CRM1/XPO1 inhibitors by CRISPR/Cas9 genome editing.

Applications

ICC/IF

Species

Human

Jasper E Neggers,Thomas Vercruysse,Maarten Jacquemyn,Els Vanstreels,Erkan Baloglu,Sharon Shacham,Marsha Crochiere,Yosef Landesman,Dirk Daelemans
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com