Anti-Ran 抗体 (ab53775)
Key features and details
- Rabbit polyclonal to Ran
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ran antibody
Ran 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ran -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide derived from Human Ran
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53775の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 23308199
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23308199 |
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
組織特異性
Expressed in a variety of tissues. -
配列類似性
Belongs to the small GTPase superfamily. Ran family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
別名
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
画像
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Western blot - Anti-Ran antibody (ab53775)All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.Left image un-treated.
Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab53775 は 9 報の論文で使用されています。
- Yoo TY & Mitchison TJ O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol 220:N/A (2021). PubMed: 33909044
- Feng H et al. MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN. Cancer Manag Res 12:2291-2300 (2020). PubMed: 32273766
- Zhong Y et al. Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Int J Biol Sci 16:1941-1953 (2020). PubMed: 32398961
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). PubMed: 28994651
- Khosravi B et al. Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD. Hum Mol Genet 26:790-800 (2017). PubMed: 28040728
- Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . PubMed: 23308199
- Lecompte S et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
- Amengual J et al. A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 25:948-59 (2011). WB ; Mouse . PubMed: 21106934
- Lobo GP et al. {beta},{beta}-Carotene Decreases Peroxisome Proliferator Receptor {gamma} Activity and Reduces Lipid Storage Capacity of Adipocytes in a {beta},{beta}-Carotene Oxygenase 1-dependent Manner. J Biol Chem 285:27891-9 (2010). WB ; Mouse . PubMed: 20573961