Anti-Ramp4-2 抗体 [EPR14338] (ab184571)
![Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.co.jp/ps/products/184/ab184571/Images/ab184571-217139-ab184571d.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (ab184571)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14338] to Ramp4-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ramp4-2 antibody [EPR14338]
Ramp4-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14338] to Ramp4-2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T, LnCaP and SW480 cell lysates; Human liver tissue; SW480 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14338 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184571の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa).
|
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/250.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/250. |
ターゲット情報
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機能
May interact with target proteins during translocation into the lumen of the endoplasmic reticulum. May protect unfolded target proteins against degradation and facilitate correct glycosylation. -
配列類似性
Belongs to the RAMP4 family. -
細胞内局在
Membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 387923 Human
- SwissProt: Q8N6R1 Human
- Unigene: 377972 Human
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別名
- Ribosome-associated membrane protein RAMP4-2 antibody
- SERP2 antibody
- SERP2_HUMAN antibody
- Stress-associated endoplasmic reticulum protein 2 antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (ab184571)
Immunofluorescent analysis of 4% paraformaldehyde-fixed SW480 cells labeling Ramp4-2 with ab184571 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.co.jp/ps/products/184/ab184571/Images/ab184571-217138-ab184571c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (ab184571)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ramp4-2 with ab184571 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.co.jp/ps/products/184/ab184571/Images/ab184571-217137-ab184571b.jpg)
Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)All lanes : Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/100000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
![Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.co.jp/ps/products/184/ab184571/Images/ab184571-217136-ab184571a.jpg)
Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/50000 dilution + 293T cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
![Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.co.jp/ps/products/184/ab184571/Images/ab184571-5-benefits-of-recombinant-antibodies.png)
Anti-Ramp4-2 antibody [EPR14338] (ab184571)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab184571 は論文での使用が確認できていません。