Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-RAGE 抗体 [EPR16849-75] (ab181293)

Submit a review Submit a question References (1)
Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
  • Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
  • Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] (ab181293)
  • Anti-RAGE antibody [EPR16849-75] (ab181293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16849-75] to RAGE
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RAGE antibody [EPR16849-75]
    RAGE 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16849-75] to RAGE

  • 由来種

    Rabbit

  • 特異性

    RAGE is typically expressed at low levels under normal physiological conditions in majority of tissues except normal lung tissue. When testing other tissues, please use lung tissue as a positive control.

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and rat lung lysates. IP: Mouse lung whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181293の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP
1/50.
特記事項
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP
1/50.

ターゲット情報

  • 機能

    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.

  • 組織特異性

    Endothelial cells.

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • DAMA 358M23.4 antibody
      • MGC2235 antibody
      • MGC22357 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycation end products antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    画像

    • Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
      Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
      All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/2000 dilution

      Lane 1 : Mouse lung lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Mouse kidney lysates
      Lane 4 : Mouse heart lysates
      Lane 5 : Mouse liver lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 1 second


      RAGE expression was predominantly high in the lung reported by PMID: 21743278.

    • Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
      Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
      All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/5000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] (ab181293)
      Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] (ab181293)

      RAGE was immunoprecipitated from 1 mg of mouse lung whole cell lysate with ab181293 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab181293 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse lung whole cell lysate 10µg (Input).

      Lane 2: ab181293 IP in mouse lung whole cell lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181293 in mouse lung whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-RAGE antibody [EPR16849-75] (ab181293)
      Anti-RAGE antibody [EPR16849-75] (ab181293)

    参考文献 (1)

    ab181293 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181293 は 1 報の論文で使用されています。

    • Liu B  et al. Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction. Oxid Med Cell Longev 2019:1703709 (2019). PubMed: 31929852

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab181293.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com