Anti-RAGE 抗体 (ab65965)
Key features and details
- Rabbit polyclonal to RAGE
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-RAGE antibody
RAGE 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAGE -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.
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ポジティブ･コントロール
- Rat lung tissue and lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65965の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
ICC: Use at a concentration of 0.5 - 1 µg/ml.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
組織特異性
Endothelial cells. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 81722 Rat
- SwissProt: Q63495 Rat
- Unigene: 9829 Rat
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別名
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab65965)Immunohistochemical analysis of paraffin-embedded Rat lung tissue sections using ab65965 at 1µg/ml
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Western blot - Anti-RAGE antibody (ab65965)Anti-RAGE antibody (ab65965) at 2 µg/ml + Rat lung tissue lysate
Predicted band size: 48 kDa
Observed band size: 48 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab65965 は 7 報の論文で使用されています。
- Meshanni JA et al. Suppression of Lung Oxidative Stress, Inflammation, and Fibrosis following Nitrogen Mustard Exposure by the Selective Farnesoid X Receptor Agonist Obeticholic Acid. J Pharmacol Exp Ther 388:586-595 (2024). PubMed: 37188530
- Yang F et al. Advanced Glycation End Products Downregulate Connexin 43 and Connexin 40 in Diabetic Atrial Myocytes via the AMPK Pathway. Diabetes Metab Syndr Obes 16:3045-3056 (2023). PubMed: 37810573
- Chen YY et al. Blocking of SGLT2 to Eliminate NADPH-Induced Oxidative Stress in Lenses of Animals with Fructose-Induced Diabetes Mellitus. Int J Mol Sci 23:N/A (2022). PubMed: 35806147
- Wächter K et al. AGE-Rich Bread Crust Extract Boosts Oxidative Stress Interception via Stimulation of the NRF2 Pathway. Nutrients 13:N/A (2021). PubMed: 34836129
- Chen YY et al. Dapagliflozin Prevents NOX- and SGLT2-Dependent Oxidative Stress in Lens Cells Exposed to Fructose-Induced Diabetes Mellitus. Int J Mol Sci 20:N/A (2019). PubMed: 31491943
- Lai S et al. Glycyrrhizin Treatment Facilitates Extinction of Conditioned Fear Responses After a Single Prolonged Stress Exposure in Rats. Cell Physiol Biochem 45:2529-2539 (2018). PubMed: 29558743
- Wang H et al. Expression of the receptor for advanced glycation end-products and frequency of polymorphism in lung cancer. Oncol Lett 10:51-60 (2015). IHC-P ; Human . PubMed: 26170976