Anti-Rag D 抗体 - N-terminal (ab187679)
Key features and details
- Rabbit polyclonal to Rag D - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rag D antibody - N-terminal -
製品の詳細
Rabbit polyclonal to Rag D - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide within Human Rag D aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_067067.1)
Database link: Q9NQL2 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187679の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 46 kDa.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 46 kDa. |
ターゲット情報
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機能
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. -
配列類似性
Belongs to the GTR/RAG GTP-binding protein family. -
細胞内局在
Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA. - Information by UniProt
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参照データベース
- Entrez Gene: 462884 Chimpanzee
- Entrez Gene: 101139071 Gorilla
- Entrez Gene: 58528 Human
- Entrez Gene: 52187 Mouse
- Omim: 608268 Human
- SwissProt: Q9NQL2 Human
- SwissProt: Q7TT45 Mouse
- Unigene: 31712 Human
see all -
別名
- bA11D8.2.1 antibody
- DKFZp761H171 antibody
- FLJ44176 antibody
see all
画像
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Western blot - Anti-Rag D antibody - N-terminal (ab187679)All lanes : Anti-Rag D antibody - N-terminal (ab187679) at 1/2000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : RRAGD knockout HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab187679 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab187679 was shown to react with Rag D in wild-type HEK-293T cells in Western blot with loss of signal observed in Rragd knockout cell line ab266287 (Rragd knockout cell lysate ab259091). Wild-type HEK-293T and Rragd knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab187679 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-Rag D antibody - N-terminal (ab187679)All lanes : Anti-Rag D antibody - N-terminal (ab187679) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Rag D antibody - N-terminal (ab187679)Immunoprecipitation. ab187679 at 1 µg/ml staining Rag D in 293T whole cell lysate immunoprecipitated using ab187679 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab187679 は 3 報の論文で使用されています。
- Fong MY et al. Cancer-secreted miRNAs regulate amino-acid-induced mTORC1 signaling and fibroblast protein synthesis. EMBO Rep 22:e51239 (2021). PubMed: 33345445
- Zhang Y et al. Amino acids and RagD potentiate mTORC1 activation in CD8+ T cells to confer antitumor immunity. J Immunother Cancer 9:N/A (2021). PubMed: 33883257
- Li J et al. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis. JCI Insight 5:N/A (2019). PubMed: 30843872