Anti-Raf1 抗体 [RNP1] (ab50858)
![Western blot - Anti-Raf1 antibody [RNP1] (ab50858)](https://www.abcam.co.jp/ps/products/50/ab50858/Images/ab50858-62560-anti-raf1-antibody-rnp1-western-blot.jpg "Western blot - Anti-Raf1 antibody [RNP1] (ab50858)")
Key features and details
- Mouse monoclonal [RNP1] to Raf1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Raf1 antibody [RNP1]
Raf1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RNP1] to Raf1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chicken, Xenopus laevis, Orangutan
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免疫原
Synthetic peptide:
GQRDSSYYWEIE
, corresponding to internal sequence amino acids 334-345 of Human Raf1 -
ポジティブ･コントロール
- Extracts and cells from A431 cell line (Human Epidermoid carcinoma)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
RNP1 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50858の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa.
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| ICC/IF |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa. |
|
ICC/IF
Use at an assay dependent dilution. |
ターゲット情報
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機能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
組織特異性
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
関連疾患
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
細胞内局在
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 5894 Human
- Entrez Gene: 100173732 Orangutan
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q5R5M7 Orangutan
- SwissProt: P09560 Xenopus laevis
- Unigene: 159130 Human
- Unigene: 5697 Xenopus laevis
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別名
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
画像
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Western blot - Anti-Raf1 antibody [RNP1] (ab50858)Lane 1 : Without Raf1 antibody [RNP1] (ab50858)
Lane 2 : Anti-Raf1 antibody [RNP1] (ab50858) at 1 µg/ml
All lanes : A431cell extract (Human Epidermoid carcinoma)
Secondary
All lanes : Goat Anti-Mouse, Peroxidase conjugate
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody [RNP1] (ab50858)](https://www.abcam.co.jp/ps/products/50/ab50858/Images/ab50858-62559-anti-raf1-antibody-rnp1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody [RNP1] (ab50858)Localisation of RAF1 protein in the cytoplasm of A431. A431 cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with Monoclonal Anti-RAF1 (ab50858) and further developed with Rabbit Anti-Mouse IgG, FITC-conjugate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab50858 は 9 報の論文で使用されています。
- Liu YF et al. Xiaozheng pill exerts an anti-mammary hyperplasia effect through Raf/ERK/ELK and HIF-1α/bFGF pathways. J Tradit Complement Med 13:600-610 (2023). PubMed: 38020551
- Li J et al. Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40). Bone Res 11:64 (2023). PubMed: 38097598
- Kwon Y et al. Non-canonical β-adrenergic activation of ERK at endosomes. Nature 611:173-179 (2022). PubMed: 36289326
- Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011
- Shao Y et al. Circular RNA circDENND2A protects H9c2 cells from oxygen glucose deprivation-induced apoptosis through sponging microRNA-34a. Cell Cycle 19:246-255 (2020). PubMed: 31878833
- Wang H et al. Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway. Aging (Albany NY) 12:16021-16034 (2020). PubMed: 32756011
- Zeng M et al. Chinese yam extract and adenosine attenuated LPS-induced cardiac dysfunction by inhibiting RAS and apoptosis via the ER-mediated activation of SHC/Ras/Raf1 pathway. Phytomedicine 61:152857 (2019). PubMed: 31035040
- Jia S et al. Overexpression of indoleamine 2, 3-dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway. Exp Ther Med 16:282-290 (2018). PubMed: 29896251
- Chen Q et al. USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. Am J Physiol Cell Physiol 315:C863-C872 (2018). PubMed: 30281322