AB202063

Anti-Rad51D 抗体 [EPR16205]

Anti-Rad51D antibody [EPR16205]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Rad51D antibody. Suitable for IP, WB, IHC-P and reacts with Human, Rat samples. Cited in 8 publications.

別名を表示する

RAD51L3, RAD51D, DNA repair protein RAD51 homolog 4, R51H3, RAD51 homolog D, RAD51-like protein 3, TRAD

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human small intestine tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human lung carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (AB202063)

Rad51D was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202063 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab202063 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab202063 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202063 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (ab202063)

Predicted band size: 35 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Western blot : Anti-Rad51D antibody [EPR16205] ab202063 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 35 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in RAD51D knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent Western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-0.1 % Tween^® 20 (TBS-T), incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysates at 20 µg

Lane 2:

Western blot - Human RAD51D knockout HCT116 cell line (<a href='/products/cell-lines/human-rad51d-knockout-hct116-cell-line-ab286478'>ab286478</a>) at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Lane 4:

Calu-3 cell lysates at 20 µg

Lane 5:

SH-SY5Y cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Unknown

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

Lane 1:

Rat kidney lysate at 10 µg

Lane 2:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16205

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rad51D also known as RAD51L3 is a protein involved in homologous recombination repair of DNA. It has a molecular weight of approximately 37 kDa. Rad51D is primarily expressed in tissues with high cell division rates such as the testis and thymus. This protein belongs to the RAD51 family which is responsible for repairing DNA double-strand breaks (DSBs) that can arise during replication or after exposure to genotoxic agents. Rad51D's role centers on promoting the exchange of DNA strands during repair processes which helps maintain genome stability.

Biological function summary

Rad51D plays a significant role in maintaining genomic integrity by facilitating homologous recombination. It interacts with Rad51 paralogs like Rad51C as part of the BCDX2 complex which also includes Rad51B and XRCC2. This complex is essential in the early steps of homologous recombination especially in forming the synaptic complex that searches for homology. The interactions among these proteins enable efficient strand invasion and exchange essential for error-free DNA repair.

Pathways

Rad51D is an important component in DNA repair and damage response pathways. It primarily functions within the homologous recombination repair pathway. In this pathway Rad51D works closely with the RAD52 epistasis group including proteins such as BRCA1 and BRCA2 which assist in the proper localization and functioning of RAD51 recombinase. Rad51D's involvement in these pathways ensures accurate and effective DNA repair preventing genomic instability that could lead to cellular dysfunction.

Rad51D mutations have been linked to a higher risk of breast and ovarian cancer. Its association with the BRCA1 and BRCA2 proteins suggests a shared pathway in hereditary breast and ovarian cancer syndromes where deficient DNA repair leads to genomic mutations and tumorigenesis. Further research continues to explore Rad51D's potential as a biomarker for cancer susceptibility and its utility in developing targeted therapies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2 : RAD51D can stimulate Holliday junction resolution by BLM.

See full target information RAD51D

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37833926

2023

RAD51D Secondary Mutation-Mediated Resistance to PARP-Inhibitor-Based Therapy in HGSOC.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Yilin Dai,Yi Gao,Ranran Chai,Chong Lu,Bing Yu,Yu Kang,Congjian Xu

Human molecular genetics 31:4241-4254 PubMed35904444

2022

Identification of new RAD51D-regulating microRNAs that also emerge as potent inhibitors of the Fanconi anemia/homologous recombination pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Hater,Katharina M Iwaniuk,Carina Leifeld,Pia Grüten,Constanze Wiek,Katharina Raba,Fan Zhang,Johannes C Fischer,Paul R Andreassen,Helmut Hanenberg,Hans-Ingo Trompeter

Nucleic acids research 50:8008-8022 PubMed35801922

2022

Strand annealing and motor driven activities of SMARCAL1 and ZRANB3 are stimulated by RAD51 and the paralog complex.

Applications

Unspecified application

Species

Unspecified reactive species

Swagata Halder,Lepakshi Ranjha,Angelo Taglialatela,Alberto Ciccia,Petr Cejka

Cancers 14: PubMed35565380

2022

The Genetic and Molecular Analyses of and Identifies Rare Variants Implicated in Hereditary Ovarian Cancer from a Genetically Unique Population.

Applications

Unspecified application

Species

Unspecified reactive species

Wejdan M Alenezi,Larissa Milano,Caitlin T Fierheller,Corinne Serruya,Timothée Revil,Kathleen K Oros,Supriya Behl,Suzanna L Arcand,Porangana Nayar,Dan Spiegelman,Simon Gravel,Anne-Marie Mes-Masson,Diane Provencher,William D Foulkes,Zaki El Haffaf,Guy Rouleau,Luigi Bouchard,Celia M T Greenwood,Jean-Yves Masson,Jiannis Ragoussis,Patricia N Tonin

Nature communications 11:3531 PubMed32669601

2020

Sequential role of RAD51 paralog complexes in replication fork remodeling and restart.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Berti,Federico Teloni,Sofija Mijic,Sebastian Ursich,Jevgenij Fuchs,Maria Dilia Palumbieri,Jana Krietsch,Jonas A Schmid,Edwige B Garcin,Stéphanie Gon,Mauro Modesti,Matthias Altmeyer,Massimo Lopes

Aging 10:1556-1574 PubMed30001218

2018

Nicotine exposure impairs germ cell development in human fetal ovaries cultured .

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Feng Cheng,Xun-Si Qin,Ze-Li Han,Xiao-Feng Sun,Yan-Ni Feng,Fan Yang,Wei Ge,Lan Li,Yong Zhao,Massimo De Felici,Shu-Hua Zou,Yi Zhou,Wei Shen

Cancer research 77:4517-4529 PubMed28646019

2017

Functionally Null Missense Mutation Associates Strongly with Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Rivera,Massimo Di Iorio,Jessica Frankum,Javad Nadaf,Somayyeh Fahiminiya,Suzanna L Arcand,David L Burk,Damien Grapton,Eva Tomiak,Valerie Hastings,Nancy Hamel,Rabea Wagener,Olga Aleynikova,Sylvie Giroux,Fadi F Hamdan,Alexandre Dionne-Laporte,George Zogopoulos,Francois Rousseau,Albert M Berghuis,Diane Provencher,Guy A Rouleau,Jacques L Michaud,Anne-Marie Mes-Masson,Jacek Majewski,Susanne Bens,Reiner Siebert,Steven A Narod,Mohammad R Akbari,Christopher J Lord,Patricia N Tonin,Alexandre Orthwein,William D Foulkes

Cancer discovery 7:984-998 PubMed28588062

2017

Secondary Somatic Mutations Restoring and Associated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Kondrashova,Minh Nguyen,Kristy Shield-Artin,Anna V Tinker,Nelson N H Teng,Maria I Harrell,Michael J Kuiper,Gwo-Yaw Ho,Holly Barker,Maria Jasin,Rohit Prakash,Elizabeth M Kass,Meghan R Sullivan,Gregory J Brunette,Kara A Bernstein,Robert L Coleman,Anne Floquet,Michael Friedlander,Ganessan Kichenadasse,David M O'Malley,Amit Oza,James Sun,Liliane Robillard,Lara Maloney,David Bowtell,Heidi Giordano,Matthew J Wakefield,Scott H Kaufmann,Andrew D Simmons,Thomas C Harding,Mitch Raponi,Iain A McNeish,Elizabeth M Swisher,Kevin K Lin,Clare L Scott

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rad51D 抗体 [EPR16205]

Anti-Rad51D antibody [EPR16205]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

Abcam product promise