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リコンビナントRabMAb

Anti-Rad51 抗体 [EPR4031] (ab109107)

Submit a review Q&A (1)References (2)
Western blot - Anti-Rad51 antibody [EPR4031] (ab109107)
  • Anti-Rad51 antibody [EPR4031] (ab109107)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4031] to Rad51
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Rad51 antibody [EPR4031]
    Rad51 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4031] to Rad51

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, A431, 293T, and K562 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4031

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109107の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

  • 組織特異性

    Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

  • 関連疾患

    Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.

  • 配列類似性

    Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.

  • ドメイン

    The nuclear localization may reside in the C-terminus (between 259 and 339 AA).

  • 翻訳後修飾

    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.

  • 細胞内局在

    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    画像

    • Western blot - Anti-Rad51 antibody [EPR4031] (ab109107)
      Western blot - Anti-Rad51 antibody [EPR4031] (ab109107)
      All lanes : Anti-Rad51 antibody [EPR4031] (ab109107) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Anti-Rad51 antibody [EPR4031] (ab109107)
      Anti-Rad51 antibody [EPR4031] (ab109107)

    参考文献 (2)

    ab109107 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109107 は 2 報の論文で使用されています。

    • Thiruvalluvan M  et al. SUMOylation controls stem cell proliferation and regional cell death through Hedgehog signaling in planarians. Cell Mol Life Sci 75:1285-1301 (2018). PubMed: 29098326
    • Peiris TH  et al. Regional signals in the planarian body guide stem cell fate in the presence of genomic instability. Development 143:1697-709 (2016). PubMed: 27013241

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I recently contacted you about a Rad51 antibody. Attached is the Rad51 sequence. The species is schmidtea mediterranea.  It contains the nucleotide  sequence and the amino acid sequence and an alignment I did with the human counterpart of it. Please let me know my options.

    Read More
    Answer

    Thank you for contacting us and for providing this essential information.

    As the identity and positives for these species are very similar I would be able to provide you with Abreview discounts should you want to test a product. When choosing which products are best for testing however I would tend to chose those which is either a polyclonal, created from a specific region of the protein (so that you can compare the homology of regions instead of the whole protein) or both.

    In this case I have looked over each of the products that we have against Rad51 and can strongly recommendAnti-Rad51 antibodies ab84304 (https://www.abcam.com/Rad51-antibody-ab84304.html) and ab109107 (https://www.abcam.com/Rad51-antibody-EPR4031-ab109107.html as the immunogen for these product had perfect homology. Further, ab84304 is a rabbit polyclonal and the peptide used is available as well.

    If you would be interested in taking advantage of 100% Abreview discount for one of these products please let me know and I will send you more information and testing code.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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