Anti-Rad51 抗体 (ab176458)
Key features and details
- Rabbit polyclonal to Rad51
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Human, Xenopus laevis, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Rad51 antibody
Rad51 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rad51 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Xenopus laevis, Chinese hamster
交差が予測される動物種: Rabbit, Cow, Dog -
免疫原
Recombinant full length protein corresponding to Human Rad51 aa 1-339.
Sequence:MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVE AVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEII QITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDR GGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQ TQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLR MLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRL YLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
Database link: Q06609 -
ポジティブ・コントロール
- WB: MCF7, CHO, NIH3T3 and Xenopus laevis eggs cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.00
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Azide and carrier free. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176458の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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IP |
1/100 - 1/1000.
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ICC/IF |
1/1000 - 1/10000.
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IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
IP
1/100 - 1/1000. |
ICC/IF
1/1000 - 1/10000. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
組織特異性
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
関連疾患
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
配列類似性
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
ドメイン
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
翻訳後修飾
Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 514749 Cow
- Entrez Gene: 403568 Dog
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Entrez Gene: 100008661 Rabbit
- Omim: 179617 Human
- SwissProt: P70099 Chinese hamster
- SwissProt: Q2KJ94 Cow
see all -
別名
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
画像
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All lanes : Anti-Rad51 antibody (ab176458) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : NIH3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 3 : CHO (Chinese hamster ovary cell line) cell lysate
Lane 4 : Xenopus laevis egg
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa -
Immunofluorescence detection of Rad51 foci formation after X-ray irradiation in GM0637 cells with ab176458 at 1/10000 dilution (left panels) and 1/1000 dilution (right panels). The secondary antibody, anti-rabbit Alexa 488 was used at 1/10000 dilution.
Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with ab176458 for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted.
The pictures were by courtesy of Prof. S. Tashiro and Dr. K. Kono at Hiroshima University.
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Anti-Rad51 antibody (ab176458) at 1/1000 dilution + Crude HeLa cell extracts at 10 µg
Secondary
Goat anti-Rabbit IgG conjugated to HRP at 1/20000 dilution
Predicted band size: 36 kDa -
ab176458 (20 µg) was incubated with 20 μg of HeLa cell extract, and precipitated with 20 μg of proteinA-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with anti-Rad51 antiserum (chicken, ab63802) at 1/1000 dilution. As secondary antibody, anti-chicken IgG antibody (rabbit) was used at 1/10000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody - ChIP Grade (ab176458)
Immunohistological staining of Rad51 protein in mouse testis using ab176458. A section of formalin fixed and paraffin embedded mouse testis was treated with ab176458 at 1/100 dilution after deparaffization and antigen retrieval. The secondary antibody, Alexa Fluor® 647conjugated anti-rabbit IgG was used at 1/1,000 dilution (top left). The sample was counter-stained with DAPI (top right) and the merged image is shown (bottom left). The white light image of the same region is shown on the bottom right.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab176458 は 21 報の論文で使用されています。
- Chansard A et al. Imaging the Response to DNA Damage in Heterochromatin Domains. Front Cell Dev Biol 10:920267 (2022). PubMed: 35721488
- Zhang Q et al. Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice. Front Nutr 9:854655 (2022). PubMed: 35836584
- Panday A et al. A modified CUT&RUN-seq technique for qPCR analysis of chromatin-protein interactions. STAR Protoc 3:101529 (2022). PubMed: 35928003
- Sharma AK et al. Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells. STAR Protoc 4:101917 (2022). PubMed: 36520630
- Arnould C et al. Analyzing Homologous Recombination at a Genome-Wide Level. Methods Mol Biol 2153:427-438 (2021). PubMed: 32840796