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リコンビナントRabMAb

Anti-Rad50 抗体 [EPR20968] - ChIP Grade (ab208019)

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Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • ChIP - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
  • Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20968] to Rad50 - ChIP Grade
  • Suitable for: WB, ChIP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Rad50 antibody [EPR20968] - ChIP Grade
    Rad50 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20968] to Rad50 - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ChIP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal brain and kidney lysates; K562, HeLa, HEK-293T, HT-29, NIH/3T3, ES-D3, C6, RAW 264.7 and PC-12 cell lysates; Mouse kidney lysate; Rat brain and spleen lysate. IHC-P: Human breast and breast carcinoma tissue; Mouse and rat testis tissue. ChIP: Chromatin prepared from HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab208019の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 154 kDa (predicted molecular weight: 153 kDa).
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 154 kDa (predicted molecular weight: 153 kDa).
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

  • 組織特異性

    Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

  • 関連疾患

    Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.

  • 配列類似性

    Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.

  • ドメイン

    The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

画像

  • Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate at 10 µg
    Lane 2 : Rat brain lysate at 10 µg
    Lane 3 : Rat spleen lysate at 10 µg
    Lane 4 : C6 (rat glial tumor cell line) whole cell lysate at 20 µg
    Lane 5 : RAW 364.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 153 kDa
    Observed band size: 154 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3: 3 minutes; Lanes 4-6: 41 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 26068589).

  • ChIP - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    ChIP - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 1% methymethanesulfonate (MMS) according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab208019 (red), and 20 µl of Protein A/G sepharose beads. 2μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). rDNA primers used are located in the region of ribosomal gene loci following the publication PMID:21029860(rDNA 1: rDNA 13017F/13068R ; rDNA 2 : rDNA 1125F/1201R ; rDNA 3 : rDNA 30409F/30566R).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human breast tissue (PMID: 15509680) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis (PMID: 10908350) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human breast carcinoma (PMID: 24642965) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 6 : ES-D3 (mouse embryonic multipotent stem Cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 153 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 8 seconds; Lanes 5-6: 24 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 26068589).

  • Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 153 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Line 1: 3 minutes; Line 2: 41 seconds.

     

  • Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)
    Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab208019 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab208019 は論文での使用が確認できていません。

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