Anti-Rad21 抗体 (ab992)
Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Rad21 antibody
Rad21 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rad21 -
由来種
Rabbit -
特異性
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
-
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
-
ポジティブ・コントロール
- WB: HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH/3T3, and TCMK-1 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP | (1) |
Use at an assay dependent concentration.
ab992 used for IP at 6 µg per reaction. |
WB | (7) |
1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Band observed at ~130 kDa. |
特記事項 |
---|
IP
Use at an assay dependent concentration. ab992 used for IP at 6 µg per reaction. |
WB
1/500 - 1/5000. Predicted molecular weight: 72 kDa. Band observed at ~130 kDa. |
ターゲット情報
-
機能
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
配列類似性
Belongs to the rad21 family. -
ドメイン
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
翻訳後修飾
Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 540966 Cow
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Entrez Gene: 314949 Rat
- Omim: 606462 Human
- SwissProt: Q3SWX9 Cow
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
see all -
別名
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
画像
-
All lanes : Anti-Rad21 antibody (ab992) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : RKO (human cell line from colon carcinoma) whole cell lysate
Lane 5 : GaMG (human cell line from glioblastoma) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 10 : TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.
Lane 1: ab992 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (207)
ab992 は 207 報の論文で使用されています。
- Dunn LEM et al. Reactivation of Epstein-Barr Virus from Latency Involves Increased RNA Polymerase Activity at CTCF Binding Sites on the Viral Genome. J Virol 97:e0189422 (2023). PubMed: 36744959
- Zhao Y et al. Multiscale 3D genome reorganization during skeletal muscle stem cell lineage progression and aging. Sci Adv 9:eabo1360 (2023). PubMed: 36800432
- Wang Z et al. An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin looping. Nat Commun 14:1208 (2023). PubMed: 36869052
- Lambuta RA et al. Whole-genome doubling drives oncogenic loss of chromatin segregation. Nature 615:925-933 (2023). PubMed: 36922594
- Wang C et al. A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth. Nat Commun 14:1598 (2023). PubMed: 36949074