Anti-RAD18 抗体 (ab186835)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RAD18 antibody
RAD18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAD18 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human RAD18 aa 1-495.
Database link: Q9NS91 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186835の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56852 Human
- Entrez Gene: 58186 Mouse
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- SwissProt: Q9QXK2 Mouse
- Unigene: 375684 Human
- Unigene: 103812 Mouse
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別名
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab186835)
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using ab186835. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab186835)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186835. Blue DAPI for nuclear staining. -
Western blot - Anti-RAD18 antibody (ab186835)All lanes : Anti-RAD18 antibody (ab186835) at 1/500 dilution
Lane 1 : Extracts from K562 cell line
Lane 2 : Extracts from MCF7 cell line
Lane 3 : Extracts from Jurkat cell line
Lane 4 : Extracts from 293T cell line
Lane 5 : Extracts from mouse liver
Predicted band size: 56 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab186835 は 2 報の論文で使用されています。
- Wu B et al. High expression of RAD18 in glioma induces radiotherapy resistance via down-regulating P53 expression. Biomed Pharmacother 112:108555 (2019). PubMed: 30798132
- Lou P et al. RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin-1ß pathway. Mol Med Rep 20:3415-3423 (2019). PubMed: 31432163