Anti-RABGGTB 抗体 (ab187717)
Key features and details
- Rabbit polyclonal to RABGGTB
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RABGGTB antibody
RABGGTB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RABGGTB -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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免疫原
Synthetic peptide within Human RABGGTB aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P53611 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat, Mouse TCMK-1 and Mouse NIH3T3 cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187717の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
ターゲット情報
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関連性
RABGGTB catalyzes the transfer of a geranyl-geranyl moiety from geranyl geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively. -
参照データベース
- Entrez Gene: 533276 Cow
- Entrez Gene: 5876 Human
- Entrez Gene: 19352 Mouse
- Entrez Gene: 25533 Rat
- Omim: 179080 Human
- SwissProt: Q5E9B3 Cow
- SwissProt: P53611 Human
- SwissProt: P53612 Mouse
see all -
別名
- Geranylgeranyl transferase type 2 subunit beta antibody
- Geranylgeranyl transferase type II subunit beta 3 antibody
- GGTase II beta antibody
see all
画像
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Western blot - Anti-RABGGTB antibody (ab187717)All lanes : Anti-RABGGTB antibody (ab187717) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RABGGTB antibody (ab187717)Detection of Human RABGGTB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: ab187717 used for IP at 6 μg per reaction (Lane 2). RABGGTB was also immunoprecipitated using an other antibody (Lane 1). Lane 3 is the Control IgG IP. For blotting immunoprecipitated RABGGTB, ab187717 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds
Lane 1 : Rabbit Anti-RABGGTB at 6 µg
Lane 2 : Anti-RABGGTB antibody (ab187717) at 6 µg
Lanes 1-2 : Immunoprecipitation of RABGGTB from 293T cell lysate
Lane 3 : Immunoprecipitation of control IgG from 293T cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab187717 は論文での使用が確認できていません。