Anti-RABGAP1 抗体 - C-terminal (ab153992)
Key features and details
- Rabbit polyclonal to RABGAP1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RABGAP1 antibody - C-terminal -
製品の詳細
Rabbit polyclonal to RABGAP1 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
-
免疫原
Recombinant fragment corresponding to a region within C terminal amino acids 720-1058 of Human RABGAP1.
-
ポジティブ･コントロール
- NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain lysates; HeLa cells; BT474 xenograft.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 121 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/20000. Predicted molecular weight: 121 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
関連性
May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition. -
細胞内局在
Cytoplasm, cytosol. Centrosome -
参照データベース
- Entrez Gene: 519260 Cow
- Entrez Gene: 23637 Human
- Entrez Gene: 227800 Mouse
- Entrez Gene: 311911 Rat
- SwissProt: Q9Y3P9 Human
- SwissProt: A2AWA9 Mouse
-
別名
- GAP and centrosome associated protein antibody
- GAPCENA antibody
- HSPC094 antibody
see all
画像
-
Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992)Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/10000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 121 kDa
7.5% SDS PAGE -
Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992)Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 121 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1 antibody - C-terminal (ab153992)Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling RABGAP1 with ab153992 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-RABGAP1 antibody - C-terminal (ab153992)Immunofluorescent analysis of methanol-fixed HeLa cells labeling RABGAP1 with ab153992 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab153992 は 2 報の論文で使用されています。
- Samarelli AV et al. Rabgap1 promotes recycling of active ß1 integrins to support effective cell migration. J Cell Sci 133:N/A (2020). PubMed: 32843574
- Kawasaki N et al. TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling. Cell Discov 4:1 (2018). PubMed: 29423269