Anti-RABEP1 抗体 (ab176578)
Key features and details
- Rabbit polyclonal to RABEP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RABEP1 antibody -
製品の詳細
Rabbit polyclonal to RABEP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Xenopus tropicalis
-
免疫原
Synthetic peptide within Human RABEP1 aa 812-862 (C terminal). The exact sequence is proprietary. NP_004694.2
Sequence:DVSEQVQRD FVKLSQTLQV QLERIRQADS LERIRAILND TKLTDINQLP ET
Database link: Q15276 -
ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176578 was affinity purified using an epitope specific to RABEP1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
関連性
RABEP1 is a Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. -
細胞内局在
Cytoplasm. Early endosome. Recycling endosome -
参照データベース
- Entrez Gene: 395930 Chicken
- Entrez Gene: 455255 Chimpanzee
- Entrez Gene: 504785 Cow
- Entrez Gene: 489451 Dog
- Entrez Gene: 9135 Human
- Omim: 603616 Human
- SwissProt: Q15276 Human
- SwissProt: O35551 Mouse
see all -
別名
- Neurocrescin antibody
- Rab GTPase binding effector protein 1 antibody
- RAB5EP antibody
see all
画像
-
Western blot - Anti-RABEP1 antibody (ab176578)All lanes : Anti-RABEP1 antibody (ab176578) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Lane 5 : NIH3T3 whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RABEP1 antibody (ab176578)Detection of RABEP1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176578 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176578 antibody which recognizes an upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab176578 は論文での使用が確認できていません。
