Rabbit IgG, polyclonal - Isotype Control (ChIP Grade)

• WB

Lab

Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (AB171870)

Please note that ab171870 in lanes 1-6 represents a negative control for Beta Actin, positively seen in lanes 7-12.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Lanes 1 and 7:

Human liver tissue lysate - total protein (ab29889) at 10 µg

Lanes 2 and 8:

Liver (Mouse) Tissue Lysate at 10 µg

Lanes 3 and 9:

Liver (Rat) Tissue Lysate at 10 µg

Lanes 4 and 10:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lanes 5 and 11:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lanes 6 and 12:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

true

Exposure time: 2min

• WB

Lab

Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (AB171870)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Lane 1:

Liver (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Rat Kidney Tissue Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

true

Exposure time: 20min