Rabbit IgG, polyclonal - Isotype Control (ab37415)
製品の概要
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製品名
Rabbit IgG, polyclonal - Isotype Control
Rabbit アイソタイプ・コントロール 製品一覧 -
特異性
This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions. -
アプリケーション
適用あり: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Protein A-purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
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別名
- rabbit isotype control
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. PubMed: 24961933
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23995768
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
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| ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20673990
|
| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. PubMed: 24961933 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23995768 |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. |
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ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990 |
画像
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Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.
PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.
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ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (279)
ab37415 は 279 報の論文で使用されています。
- Zecher BF et al. HLA-DPA1*02:01~B1*01:01 is a risk haplotype for primary sclerosing cholangitis mediating activation of NKp44+ NK cells. Gut 73:325-337 (2024). PubMed: 37788895
- Zudeh G et al. PACSIN2 as a modulator of autophagy and mercaptopurine cytotoxicity: mechanisms in lymphoid and intestinal cells. Life Sci Alliance 6:N/A (2023). PubMed: 36596605
- Li YR et al. Long non-coding RNA MRPL23-AS1 suppresses anoikis in salivary adenoid cystic carcinoma in vitro. Oral Dis 29:1588-1601 (2023). PubMed: 35175670
- Sabaté-Pérez A et al. Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis. Autophagy 19:904-925 (2023). PubMed: 35947488
- Chen HA et al. Senescence Rewires Microenvironment Sensing to Facilitate Antitumor Immunity. Cancer Discov 13:432-453 (2023). PubMed: 36302222