AB199376

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)

RabMAb
Advanced Validation
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(20 Publications)

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) Suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P, Flow Cyt, WB and reacts with samples. Cited in 20 publications.

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Immunohistochemistry analysis of mouse colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on mouse colon tissue was observed.

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (PE). Please refer to ab209478 for protocol details.

Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195884, Rat monoclonal [YOL1/34] to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeled with ab199376 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing no staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab199376 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Immunohistochemistry analysis of rat colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on rat colon tissue was observed.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Immunohistochemistry analysis of human colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on human colon tissue was observed.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647). Please refer to ab199093 for protocol details.

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199093 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488). Please refer to ab199091 for protocol details.

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376)

Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling KCTD5 with ab194825 at 1/70 dilution (red) compared with ab199376 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab199376 detects no signal in FC.

Note : ab199376 detects no signal in Flow cytometry.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25A

アイソタイプ

IgG

キャリアフリー

Yes

アプリケーション

IHC-P, ChIP-seq, ICC/IF, IP, ChIC/CUT&RUN-seq, WB, Flow Cyt

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/70", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "ChIPseq-species-checked": "", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

ab199376 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Endotoxin level: <1EU/mg (based on Lumulus Amebocyte lysate assay).

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

style

left-column

文献 (20)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 17:113-128 PubMed38384996

2024

Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chuangui Chen,Jinsheng Ding,Zhao Ma,Yongjie Xie,Linhua Zhang,Dunwan Zhu

Cell death discovery 8:340 PubMed35906204

2022

Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina E Grieco,Noemi Brusco,Daniela Fignani,Laura Nigi,Caterina Formichi,Giada Licata,Lorella Marselli,Piero Marchetti,Laura Salvini,Laura Tinti,Agnese Po,Elisabetta Ferretti,Guido Sebastiani,Francesco Dotta

Theranostics 12:3024-3044 PubMed35547761

2022

Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Mahendra D Chordia,Yi Zhang,Hui Zong,Dongfeng Pan,Zhiyi Zuo

Scientific reports 11:14849 PubMed34290311

2021

CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1.

Applications

Unspecified application

Species

Unspecified reactive species

Sibel Durlanik,Katrin Fundel-Clemens,Coralie Viollet,Heinrich J Huber,Martin Lenter,Kerstin Kitt,Stefan Pflanz

Frontiers in endocrinology 11:596898 PubMed33281748

2020

SARS-CoV-2 Receptor Angiotensin I-Converting Enzyme Type 2 (ACE2) Is Expressed in Human Pancreatic -Cells and in the Human Pancreas Microvasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Fignani,Giada Licata,Noemi Brusco,Laura Nigi,Giuseppina E Grieco,Lorella Marselli,Lut Overbergh,Conny Gysemans,Maikel L Colli,Piero Marchetti,Chantal Mathieu,Decio L Eizirik,Guido Sebastiani,Francesco Dotta

Journal of cellular and molecular medicine : PubMed33094923

2020

Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor β and S100A8/9 up-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkun Wang,Ge Zheng,Guangjian Li,Mengkun Wang,Zhanchuan Ma,Huimin Li,Xiang-Yang Wang,Huanfa Yi

Developmental cell 53:9-26.e4 PubMed32197068

2020

Glycolysis-Independent Glucose Metabolism Distinguishes TE from ICM Fate during Mammalian Embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangtao Chi,Mark S Sharpley,Raghavendra Nagaraj,Shubhendu Sen Roy,Utpal Banerjee

International journal of molecular sciences 20: PubMed31491902

2019

Decreased Level of Neurotrophic Factor Neuritin 1 in Women with Ovarian Endometriosis after Receiving Gonadotropin-Releasing Hormone Agonist Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Endah Rahmawati,Wei-Chung Vivian Yang,Yen-Ping Lei,Pawan Kumar Maurya,Huei-Wen Chen,Chii-Ruey Tzeng

Molecular cell 76:70-81.e9 PubMed31445886

2019

Regulation of Co-transcriptional Pre-mRNA Splicing by mA through the Low-Complexity Protein hnRNPG.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine I Zhou,Hailing Shi,Ruitu Lyu,Adam C Wylder,Żaneta Matuszek,Jessica N Pan,Chuan He,Marc Parisien,Tao Pan

European journal of pharmacology 861:172613 PubMed31421089

2019

Novel insights into the role of LRRC8A in ameliorating alveolar fluid clearance in LPS induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Huiran Zhang,Yani Liu,Honglin Li,Jingwen Li,Yuan Luo,Xixin Yan

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)