AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Advanced Validation
Recombinant
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(617 Publications)

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is a rabbit monoclonal antibody used in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ChIP.

- Biophysical QC for unrivalled batch-batch consistency
- Over 610 publications

41 Images

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

Conjugated versions are available for this clone : Alexa Fluor^® 488 (ab199091), Alexa Fluor^® 647 (ab199093), R-PE (ab209478), APC (ab232814).

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab315240 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : SH-SY5Y.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CKAP4 with ab302539 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized (SMAD2 KO HeLa (human cervical adenocarcinoma epithelial cell) treated with 20ng/ml TGF-β1 for 1h (Red) and untreated SMAD2 KO HeLa (dotted red)) (Left) / (Parental HeLa treated with 20ng/ml TGF-β1 for 1h (Red) and untreated HeLa (dotted red)) (Right) cells labelling Smad2 (phospho S465 + S467) with ab316117 at 1/5000 dilution (0.01 ug)/Red and Dotted Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with ab300400 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : MCF7 (PMID : 33652767).

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / MJ [G11] (human Peripheral blood T lymphoblast, Right) cells labelling RANK with ab325015 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : Daudi.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling WDR5 with ab307664 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling TRIM8 with ab316149 at 1/50 dilution (1 ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling BASP1 with ab312322 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : K-562 (PMID : 14701728).

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling RANK with ab325015 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells.
Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell, Left) / ARPE-19 (human eye epithelial cell, Right) cells labelling Fibrillin 1 with ab315384 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : T-47D.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Left) / BxPC-3 (human pancreas adenocarcinoma epithelial cell, Right) cells labeling Lewis a,b carbohydrate with ab322960 at 1/100 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : HepG2

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling HLA E with ab324677 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Low expression : HEK-293.
Cells were stained on ice to avoid internalization.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling HLA E with ab324677 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were stained on ice to avoid internalization.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with ab313773 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with CD56 conjugated to BV421. Gated on viable cells.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab313773 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Jurkat (PMID : 19525985). Gated on viable cells.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SIRT1 KO A549(Human SIRT1 knock out human lung carcinoma epithelial cell, Green) / A549 (Magenta) cells labelling SIRT1 with ab110304 at 1/1000 dilution (0.1µg)/Red followed by a Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black and Grey) isotype control.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Molt-4 (human lymphoblastic leukemia T lymphoblast, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling Cytokeratin 18 with ab315096 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : Molt-4.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) treated with 1.25% DMSO for 5days) (Red) / untreated HL-60 (Green) cells labelling PADI4 / PAD4 with ab315269 at 1/5000 dilution (0.01 ug)/Red and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling HLA E with ab324677 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Low expression : K-562.
Cells were stained on ice to avoid internalization.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Parental HeLa (EDWT03) (Green) / PFKP KO HeLa (ED030126) (Magenta) cells labelling PFKP with ab325016 at 1/500 dilution (0.1ug) (Magenta) and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labeling Cytokeratin 19 with ab323562 at 1/1000 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : SH-SY5Y.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling Granzyme A with ab321992 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD14 conjugated to Pacific blue.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) (Right) / 293T (human embryonic kidney epithelial cell) (Left) cells labelling Granzyme A with ab321992 at 1/500 dilution (0.01ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : 293T.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD56 conjugated to PE.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD8a conjugated to Pacific blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Immunohistochemical analysis of formalin fixed paraffin embedded human heart labelling Rb IgG with ab172730 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab172730 Rabbit IgG, monoclonal [EPR25A] - Isotype Control was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling Rb IgG with ab172730 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab172730 Rabbit IgG, monoclonal [EPR25A] - Isotype Control was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (human lung carcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling CARD9 with ab321988 at 1/5000 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081 at 1/5000 was used as the secondary antibody.

Low expression : A549.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A375 (human malignant melanoma epithelial cell, Left) / SK-MEL-28 (human malignant melanoma cell, Right) cells labelling ASAH1 with ab322109 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : A375 (PMID : 26553872).

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling TRBP with ab324950 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Immunohistochemical analysis of formalin fixed paraffin embedded human liver labelling Rb IgG with ab172730 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab172730 Rabbit IgG, monoclonal [EPR25A] - Isotype Control was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells.
Cells were co-stained with anti-CD56 conjugated to Brilliant Violet 421.

Flow Cyt

Lab

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of KARPAS-299 (human T cell lymphoma cell) (Right) / HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling HLA DR + DP + DQ with ab7856 at 1/1000 dilution (0.1&micor;g)/Red followed by a secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) used at a 1/5000 dilution compared with isotype control Rabbit monoclonal IgG (ab172730) / Black and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

Gated on viable cells.
Negative control : HepG2.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IL8 KO PC-3 (IL-8 knock out human prostate adenocarcinoma epithelial cell) treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours (Megenta) or untreated with IL8 KO PC-3(Green)(Right) / PC-3 treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours(Megenta) or untreated with PC-3(Green)(Left) cells labelling IL-8 with ab242515 at 1/500 dilution (0.1µg) followed by a Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with a Influenza A virus PB1 protein expression vector containing a myc tag cells labelling Influenza A virus PB1 protein with ab313887 at 1/500 dilution (0.1 µg)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SIRT1 with ab110304 at 1/1000 dilution (0.1µg)/Red followed by a Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labelling ETS1 with ab307672 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HepG2 (PMID : 30790340)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LNCaP (human prostate carcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling TCF7 with ab315392 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : LNCaP.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NSD3 with ab300489 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling MAVS with ab290729 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25A

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ChIP-seq, IHC-P, ChIC/CUT&RUN-seq, IP, WB, Flow Cyt (Intra), ICC/IF, Flow Cyt

applications

style

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "0.5-2 µg", "ChICCUTRUNseq-species-notes": "", "ChIPseq-species-checked": "", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

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文献 (617)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 29: PubMed38099337

2023

Exploring the regulatory role of Linc00893 in asthenozoospermia: Insights into sperm motility and SSC viability.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Lu,Dongchuan Xu,Liqiang Zhao,Hailing Ruan,Anguo Wang,Jiajia Hu,Meifang Xiao,Weiying Lu

MedComm 4:e464 PubMed38107057

2023

STAT4 facilitates PD-L1 level via IL-12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Zhou,Feng Wan,Li Wang,Cheng Peng,Ruizhen Huang,Fu Peng

Journal of translational medicine 21:910 PubMed38098048

2023

Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Jun Liu,Minmei Guo,Tzu-Cheng Sung,Ting Wang,Tao Yu,Zeyu Tian,Guoping Fan,Wencan Wu,Akon Higuchi

Neoplasma 70:659-669 PubMed38053376

2023

TRIM11 regulated by m6A modification promotes the progression of cervical cancer by PHLPP1 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Zhang,Yi Tang,Jing Zhao,Jing Yang,Yan Chen,Yingping Gong,Shengjun Meng,Chuqiang Shu

Neoplasma 70:597-609 PubMed38053379

2023

RNASEH1-AS1 induced by H3K27ac stabilizes ANXA2 mRNA to promote the progression of colorectal cancer through recruiting BUD13.

Applications

Unspecified application

Species

Unspecified reactive species

Shengwei Zhuang,Weihong Lu,Lianjun Shen,Zhekun Huang,Xiuping Zhang,Yong Zhang

PeerJ 11:e16553 PubMed38077417

2023

pH-sensing G protein-coupled orphan receptor is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progression.

Applications

Unspecified application

Species

Unspecified reactive species

Nazir M Khan,Martha E Diaz-Hernandez,William N Martin,Bhakti Patel,Samir Chihab,Hicham Drissi

Nature genetics 56:100-111 PubMed38049663

2023

The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandipan Brahma,Steven Henikoff

Journal of experimental & clinical cancer research : CR 42:330 PubMed38049814

2023

Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanshan Yao,Chunji Chen,Jing Wang,Haojie Xuan,Xiuxiu Chen,Zheng Li,Fuzhi Yang,Bin Wang,Siyun Lin,Saitian Li,Dongfang Tang,Libao Gong,Wen Gao

Journal of nanobiotechnology 21:459 PubMed38037135

2023

Biomimetic composite hydrogel promotes new bone formation in rat bone defects through regulation of miR-19b-3p/WWP1 axis by loaded extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Rongkang Guo,Chaohan Wu,Fan Liu,Tianhua Dong,Tao Zhang

Scientific reports 13:20262 PubMed37985889

2023

Association of CD4-positive cell infiltration with response to vedolizumab in patients with ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Miyazaki,Namiko Hoshi,Tsukasa Ishida,Chiharu Nishioka,Sachiko Ouchi,Daisuke Shirasaka,Tomoo Yoshie,Yoshinori Munetomo,Yoshio Sakamoto,Tatsuya Osuga,Saori Matsui,Toshiki Hyodo,Tamami Denda,Daisuke Watanabe,Makoto Ooi,Yuzo Kodama

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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chicCutRunSequencingBooklet

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control