AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Advanced Validation
Recombinant
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(1211 Publications)

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is a rabbit monoclonal antibody used in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ChIP-seq, ChIC/CUT&RUN-seq.

- Biophysical QC for unrivalled batch-batch consistency
- Over 610 publications

41 Images

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

Conjugated versions are available for this clone : Alexa Fluor^® 488 (ab199091), Alexa Fluor^® 647 (ab199093), R-PE (ab209478), APC (ab232814).

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram showing top CD3⁺ human PBMCs (positive cells) and bottom CD19⁺ human PBMCs (negative cells) stained with ab325911 (red line). Cells were fixed and permeabilised with BD Cytofix/Cytoperm for 20 min. Cells were incubated for 30 min on ice in 1× PBS containing 10 μg/ml human IgG and 10% normal goat serum to block Fc receptors and non‑specific protein-protein interactions, followed by the antibody ab325911 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1×10⁶ cells in 100 μl at 0.2 μg/ml) for 30 min at 22°C. The cells were simultaneously stained with CD3 and CD19.

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control, used at the same concentration and under the same conditions as the primary antibody. An unlabelled sample (without incubation with the primary antibody, blue line) was also used as a control. The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), preadsorbed, was incubated at 1/4000 for 30 min at 22°C.

Acquisition of >30, 000 events was performed using a 50 mW blue laser (488 nm) and a 525/40 bandpass filter. Events were gated on either CD3⁺ or CD19⁺ cells.

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 10uM MG-132 for 4 hours (Green) and Untreated control (Magenta) cells labelling Nrf2 with ab325240 at 1/500 dilution (0.1ug) / Green and Red compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of A549 (human lung carcinoma epithelial cell, Green and black) and CD38 KO A549 (CA050605) (Magenta and grey) cells labelling CD38 with ab326878 at 1/50 dilution (1ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) / Black and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram of WERI-Rb-1 (human Eye Retina grape-like clusters of round cells) labelling GPC2 with ab324378 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details, Goat anti-Rabbit IgG (Alexa Fluor^® 647 (ab150083), at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell) (Green and black) and KEAP1 KO HAP1(HO010289) (Magenta and grey) cells labelling with ab326338 at 1/500 dilution (0.1ug), compared with a Rabbit monoclonal IgG (ab172730) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling with ab326720 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with CD11c conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) treated with 20µM nocodazole for 24 hours (Right) / Untreated control (Left) cells labelling KMT6 / EZH2 (phos Thr487) with ab300567 at 1/500 dilution (0.1ug)/ Left and Right (Red) compared with an isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD38 with ab326878 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) TF-1 (human erythroleukemia erythroblast, Right) cells labelling Leptin Receptor with ab325970 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : Daudi.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram of Negative control : HeLa (human cervical adenocarcinoma epithelial cells, Left) and IMR-32 (human neuroblastoma neuroblasts, Right) labelling GPC2 with ab324378 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details, Goat anti-Rabbit IgG (Alexa Fluor^® 647 (ab150083), at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Huh7 (human hepatocellular carcinoma epithelial cell, Right) and K-562 (human chronic myelogenous leukemia lymphoblast, Left) cells labelling Integrin alpha 1 with ab326330 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Low expression : K-562. Gated on viable cells.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC isolated CD14+ monocytes treated with 1000IU/ml IFNα for 24hours (Lower left and right) labelling Sialoadhesin/CD169 with ab183356 at 1/50 dilution (1ug)/ (Right) compared with a Rabbit monoclonal IgG (ab172730) / Left - Isotype Control (Left). Untreated Human PBMC isolated CD14+ monocytes (Upper left and right).

Cells blocked with 20ug/ml Human IgG.

Cells were surface stained with anti-CD14 conjugated to BV421. Then fixed with 4% PFA for 10 min followed by intracellular staining with rabbit IgG or our antibody.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of LS513 (human Large intestine Cecum epithelial cell, Right) Daudi (human Burkitt's lymphoma lymphoblast, Left) cells labelling Tspan-8 with ab326570 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : Daudi.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells. Cells were co-stained with CD4 conjugated to APC/Fire™ 750.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of NCI-H1299 (human lung carcinoma epithelial cell, Right) and 293T (human embryonic kidney epithelial cell, Left) cells labelling TNF Receptor I with ab326935 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : 293T.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left ) / PC-3 (human prostate adenocarcinoma epithelial cell, Right ) cells labelling FOXA1 + FOXA2 + FOXA3 with ab317046 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : HCT 116

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Unstimulated HL-60 (Human acute promyelocytic leukemia promyeloblast) (Lower) HL-60 stimulated with 1.25% DMSO for 5 days (Upper) cells labelling CD66b with ab325777 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram showing wild-type A-549 (green line) and SHMT2 knockout A-549 stained with ab316328 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab316328) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C.

Isotype control antibody was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control in A-549 WT cells (black line) and A-549-SHMT2 KO cells (grey line), at the same conditions as the primary antibody.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) (Right) HeLa (human cervical adenocarcinoma epithelial cell) (Left) cells labelling CD5 with ab325643 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Negative control : HeLa (PMID : 15187131).

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T cells transfected with a mouse Cd244 expression vector containing a myc-His-tag® (Middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling 2B4 with ab325752 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 4% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD5 with ab325643 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti human CD3 conjugated to Alexa Fluor®647.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells. Cells were co-stained with CD3 conjugated to Brilliant Violet 421.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of BT-549 (human Breast, Mammary gland epithelial, Right) and SH-SY5Y (human neuroblastoma epithelial cell, Left) cells labelling TNF Receptor I with ab326935 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : SH-SY5Y.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T cells transfected with a human CD45RB expression vector containing a HA-Myc-tag® (Upper left and middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Upper right) 293T cells transfected with a human CD45RAB expression vector containing a HA-Myc-tag® (Lower) 293T cells transfected with a human CD45RO expression vector containing a HA-Myc-tag® (Lower) 293T cells transfected with a human CD45RC expression vector containing a HA-Myc-tag® (Lower) 293T cells transfected with a human CD45RA expression vector containing a HA-Myc-tag® (Lower) cells labelling with ab326720 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 4% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell) (Green and black) IL17RA KO HAP1(HO010217) (Magenta and grey) cells labelling IL-17RA Receptor with ab325606 at 1/500 dilution (0.1ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) / Black and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SU-DHL-1 (human histiocytic lymphoblast-like cell, Left) and karpas-299 (human T cell lymphoma cell, Right) cells labelling ROR gamma with ab325651 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : SU-DHL-1(PMID : 29588546).

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling IL-17RA Receptor with ab325606 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : MOLT-4.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human purified neutrophils cells labelling IL-17RA Receptor with ab325606 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Integrin alpha 1 with ab326330 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells. Cells were co-stained with CD3 conjugated to Alexa Fluor®488.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human purified neutrophils cells labelling Liver Arginase with ab326945 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCR2 with ab325787 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD56 conjugated to Brilliant Violet 421. Gated on viable lymphocyte cells.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD4 conjugated to Alexa Fluor®647. Gated on viable lymphocyte cells.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD19 conjugated to PE/CY7. Gated on viable lymphocyte cells.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human purified neutrophils cells labelling CXCR2 with ab325787 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC isolated CD14+ monocytes treated with IFNa (1000 IU/ml ) for 24 hours (Lower left and right) Human PBMC (human peripheral blood mononuclear cell) isolated CD14+ monocytes (Upper left and right) cells labelling Sialoadhesin/CD169 with ab326438 at 1/50 dilution (1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD14 conjugated to Brilliant Violet 421.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) SH-SY5Y (human neuroblastoma epithelial cell, Left) cells labelling with ab326720 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Negative control : SH-SY5Y.

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human neutrophils cells labelling CD66b with ab325777 at 1/5000 (0.01ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling with ab325712 at 1/5000 dilution (0.01ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor®488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : 293T.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25A

アイソタイプ

IgG

キャリアフリー

アプリケーション

IHC-P, Flow Cyt (Intra), ChIC/CUT&RUN-seq, Flow Cyt, WB, IP, ChIP-seq, ICC/IF

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "0.5-2 µg", "ChICCUTRUNseq-species-notes": "", "ChIPseq-species-checked": "", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt, ICC/IF, IHC-P, IP, and WB.

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was first used in a scientific publication in 2014 and has been cited over 617 times in peer reviewed journals. It's performance in Western blot, immunofluorescence, IHC and ChIP in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is sold in 200 µg selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR25A - ab210849.

Antibody clone EPR25A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 488, Alexa Fluor^® 647, HRP, Alexa Fluor^® 45, Alexa Fluor^® 594, Alexa Fluor^® 555, PE, Alexa Fluor^® 568, PerCP, FITC, APC, Biotin, Alexa Fluor^® 75 (ab19991, ab19993, ab19957, ab2815, ab28568, ab28569, ab29478, ab29613, ab22217, ab223339, ab232814, ab3273, ab32286).

One of the top cited IgG Isotype control antibodies in the market with >1000 citations. IgG Isotype Control antibodies are essential for multiple applications. Isotype Control ab172730 is suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P,Flow Cyt, WB. This negative control antibodies help distinguish specific antibody binding from non-specific interactions, ensuring reliable results. Optimize your research with high-quality IgG Isotype Control antibodies for precise data interpretation

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

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文献 (1211)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation (London, England) 22:41 PubMed41057834

2025

Baicalein inhibits mycobacterium tuberculosis-induced macrophage M1 polarization depending on the regulation of YY1/RAB10/TLR4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhou,Shuanghua Chen,Linfei Shu,Qian Wang,Ting Yuan,Yangjing Ou,Ling Qing,Xiaojin He

Oncology research 33:3023-3040 PubMed41050080

2025

Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Wang,Xinzhi Yang,Lvbiao Lin,Jianbo Yu,Jie Mao

Journal of cell communication and signaling 19:e12032 PubMed41050263

2025

Emerging role of HIC1 in prostate cancer progression and therapeutic response: A novel perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Dun Xue,Long Tan,Fengshuai Yang,Xiaolan Tian,Qian Zuo,Xinghui Wu

Acta pharmaceutica Sinica. B 15:4751-4771 PubMed41049757

2025

as a super-enhancer-regulated gene drives T-ALL progression HIF1A deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Xu,Zimu Zhang,Juanjuan Yu,Tongting Ji,Jia Cheng,Xiaodong Fei,Xinran Chu,Yanfang Tao,Yan Xu,Pengju Yang,Wenyuan Liu,Gen Li,Yongping Zhang,Yan Li,Fenli Zhang,Ying Yang,Bi Zhou,Yumeng Wu,Zhongling Wei,Yanling Chen,Jianwei Wang,Di Wu,Xiaolu Li,Yang Yang,Guanghui Qian,Hongli Yin,Shuiyan Wu,Shuqi Zhang,Dan Liu,Jun-Jie Fan,Lei Shi,Xiaodong Wang,Shaoyan Hu,Jun Lu,Jian Pan

World journal of surgical oncology 23:350 PubMed41024019

2025

SUZ12 knockdown restrains the proliferation, migration, and invasion of oral tongue squamous cell carcinoma through inhibiting DNMT1-mediated ZNF582 promoter methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangwei Kong,Yicheng Cheng,Lin Zhang,Wei Yin,Chenchen Wang,Zhan'ao Wu

Hereditas 162:186 PubMed40999468

2025

The FOXD1/NAT10 positive feedback loop drives nasopharyngeal carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Leifeng Liu,Qizhu Chen,Yiling Li,Weihao Wu,Feng Jiang,Haitao Qiu

Journal of orthopaedic surgery and research 20:831 PubMed40993725

2025

USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Liu,Jin Zhang,Jichang Bai,Kuanzhi Liu

eLife 13: PubMed40996791

2025

Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Bonnifet,Sandra Sinnassamy,Olivia Massiani-Beaudoin,Philippe Mailly,Heloise Monnet,Damarys Loew,Berangere Lombard,Nicolas Servant,Rajiv L Joshi,Julia Fuchs

JCI insight 10: PubMed40985894

2025

FXR shapes an immunosuppressive microenvironment in PD-L1lo/- non-small cell lung cancer by upregulating HVEM.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Xu,Bin Shang,Hancheng Wu,Xiuye Jin,Junren Wang,Jing Li,Daowei Li,Bin Liang,Xingguang Wang,Lili Su,Wenjie You,Shujuan Jiang

Journal of cell communication and signaling 19:e70046 PubMed40977851

2025

Tri-domain proteins 27 alleviates ischemia-reperfusion injury-induced acute kidney injury by promoting Gli-like transcription factor 1 expression via the inhibition of polycomb repressive complex 2 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Chongxiang Xiong,Haishan Chen,Baoting Su,Li Zhang,Jingxiang Hu,Qiaowen Wang,Shougang Zhuang

View all publications

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chicCutRunSequencingBooklet

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit IgG, monoclonal [EPR25A] - Isotype Control