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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Rab9 抗体 [EPR13272] - BSA and Azide free (ab236127)

Submit a review Submit a question References (2)
Western blot - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
  • Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13272] to Rab9 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Rab9 antibody [EPR13272] - BSA and Azide free
    Rab9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13272] to Rab9 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HepG2 cells. WB: MDA-MB-231, MCF7 and HeLa cell lysates.

  • 特記事項

    ab236127 is the carrier-free version of ab179815.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab236127の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

ターゲット情報

画像

  • Western blot - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Western blot - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB9A knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179815).

    Lanes 1-4: Merged signal (red and green). Green - ab179815 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab179815 Anti-Rab9 antibody [EPR13272]  was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used.  Wild-type and Rab9 knockout samples were subjected to SDS-PAGE.  ab179815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescent staining of HepG2 cells labeling Rab9 with unpurified ab179815 at 1/50 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab236127 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab236127 は 2 報の論文で使用されています。

  • Lin L  et al. Mito-TEMPO, a Mitochondria-Targeted Antioxidant, Improves Cognitive Dysfunction due to Hypoglycemia: an Association with Reduced Pericyte Loss and Blood-Brain Barrier Leakage. Mol Neurobiol 60:672-686 (2023). PubMed: 36357613
  • Lin L  et al. Severe Hypoglycemia Contributing to Cognitive Dysfunction in Diabetic Mice Is Associated With Pericyte and Blood-Brain Barrier Dysfunction. Front Aging Neurosci 13:775244 (2021). PubMed: 34899278

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