AB193360

Anti-RAB7B 抗体 [EPR15727]

Anti-RAB7B antibody [EPR15727]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal RAB7B antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 4 publications.

別名を表示する

Ras-related protein Rab-7b, RAB7B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] (AB193360)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] (AB193360)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RAB7B antibody [EPR15727] (AB193360)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

U937 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Supplier Data

Western blot - Anti-RAB7B antibody [EPR15727] (AB193360)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution

All lanes:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15727

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/270", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RAB7B also known as Ras-related protein Rab-7b is a small GTPase belonging to the Ras superfamily. This protein weighs approximately 23 kDa. Researchers find RAB7B in various tissues including the immune and the central nervous system. It plays a direct role in intracellular protein trafficking and vesicular transport moving proteins and other molecules from one organelle to another within cells. Through its association with specific membrane compartments RAB7B assists in maintaining the dynamic balance of vesicle budding transport and fusion regulating endosomal sorting.

Biological function summary

The functions of RAB7B include its contribution to lysosomal biogenesis and endosome-to-lysosome trafficking. It is part of a complex known as the endosomal transport modulation machinery where it interacts with multiple effector proteins to translocate cargo to the lysosome for degradation or recycling. In immune cells RAB7B helps modulate immune response by controlling the degradation of signaling receptors. This function makes it essential for cellular homeostasis and adaptive immune responses influencing antigen processing.

Pathways

The interaction of RAB7B with other members of the Rab GTPase family situates it in important cellular pathways like the endocytic pathway and autophagy. In the endocytic pathway it coordinates with proteins like RAB5A transitioning early endosomes to late endosomes. The action in autophagy involves interaction with other autophagic regulators to aid in autophagosomal-lysosomal fusion influencing how cells handle stress and remove damaged components.

Abnormal RAB7B expression has been linked to neurodegenerative diseases like Alzheimer's and immune disorders such as rheumatoid arthritis. Its interaction with other proteins like LRRK2 in Parkinson's disease implicates it in pathways that lead to neuronal damage and inflammation. In rheumatoid arthritis RAB7B influences immune cell signaling and cytokine release contributing to autoimmune responses. Understanding its associations aids in developing therapeutic strategies targeting the molecular functions of RAB7B in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.

See full target information Ras-related protein Rab-7b

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:680-693 PubMed40069506

2025

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Long Liang,Xinwei Kuang,Yi He,Lin Zhu,Poyee Lau,Xin Li,Dingan Luo,Lan Gong,Wenbin Zhou,Fanglin Zhang,Xiaowei Liang,Zhuofeng Li,Bin Hu,Dandan Liu,Tao Ding,Hui Li,Shuang Zhao,Juan Su,Mien-Chie Hung,Jing Liu,Hong Liu,Xiang Chen

Cell reports. Medicine 5:101357 PubMed38237597

2024

Benzosceptrin C induces lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting DHHC3.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Wang,Jinxin Wang,Dianping Yu,Qing Zhang,Hongmei Hu,Mengting Xu,Hongwei Zhang,Saisai Tian,Guangyong Zheng,Dong Lu,Jiajia Hu,Mengmeng Guo,Minchen Cai,Xiangxin Geng,Yanyan Zhang,Jianhua Xia,Xing Zhang,Ang Li,Sanhong Liu,Weidong Zhang

American journal of cancer research 13:1744-1765 PubMed37293175

2023

DDX3 suppresses hepatocellular carcinoma progression through modulating the secretion and composition of exosome.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Yuan Su,Chi-Hong Chao,Hsiang-Yu Hsu,Hao-Kang Li,Yi-Ling Wang,Yan-Hwa Wu Lee,Ru-Tsun Mai

EMBO molecular medicine 15:e16104 PubMed36722641

2023

Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Brundu,Virginia Napolitano,Giulia Franzolin,Ettore Lo Cascio,Roberta Mastrantonio,Gabriele Sardo,Eliano Cascardi,Federica Verginelli,Sergio Sarnataro,Gennaro Gambardella,Alberto Pisacane,Alessandro Arcovito,Carla Boccaccio,Paolo M Comoglio,Enrico Giraudo,Luca Tamagnone

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB7B 抗体 [EPR15727]

Anti-RAB7B antibody [EPR15727]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] (AB193360)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

Western blot - Anti-RAB7B antibody [EPR15727] (AB193360)

Western blot - Anti-RAB7B antibody [EPR15727] (AB193360)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise