Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-RAB7 (phospho S72) 抗体 [MJF-R38-1] (ab302494)

Submit a review Submit a question References (2)
Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
  • Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
  • Dot Blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
  • Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R38-1] to RAB7 (phospho S72)
  • Suitable for: WB, Dot blot, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RAB7 (phospho S72) antibody [MJF-R38-1]
    RAB7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [MJF-R38-1] to RAB7 (phospho S72)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blot, IHC-Pmore details
    適用なし: Flow Cyt (Intra) or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T co-transfected with a human LRRK1(K746G mutation) expression vector containing a GFP-tag and a human RAB7 expression vector containing an HA-tag, whole cell lysate. GFP-LRRK1 stably expressed HEK-293T treated with 100 ng/ml PMA for 30 minutes, whole cell lysate. Dot Blot: RAB7 phospho S72 peptides. IHC-P: Human stomach tissue. Human bladder cancer tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This antibody was developed with support from The Michael J. Fox Foundation.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302494の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 25,23 kDa.
Dot blot
1/1000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 25,23 kDa.
Dot blot
1/1000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt (Intra) or IP.

ターゲット情報

  • 機能

    Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.

  • 組織特異性

    Widely expressed; high expression found in skeletal muscle.

  • 関連疾患

    Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 細胞内局在

    Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CMT2B antibody
      • PRO2706 antibody
      • PSN antibody
      • RAB7, member RAS oncogene family antibody
      • RAB7A antibody
      • RAB7A, member RAS oncogene family antibody
      • RAB7A_HUMAN antibody
      • Ras associated protein RAB7 antibody
      • Ras related protein Rab7 antibody
      • Ras related protein Rab7a antibody
      • Ras-related protein Rab-7a antibody
      see all

    画像

    • Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      All lanes : Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (ab302494) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) co-transfected with a human LRRK1(D1409A mutation) expression vector containing a GFP-tag and a human RAB7 expression vector containing an HA-tag, whole cell lysate
      Lane 2 : HEK-293T co-transfected with a human LRRK1(K746G mutation) expression vector containing a GFP-tag and a human RAB7 expression vector containing an HA-tag, whole cell lysate
      Lane 3 : Untreated GFP-LRRK1 stably expressed HEK-293T whole cell lysate
      Lane 4 : GFP-LRRK1 stably expressed HEK-293T treated with 100 ng/ml PMA for 30 minutes, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 23,25 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking / Diluting buffer: 5% NFDM/TBST.

      The D1409A mutation of LRRK1 results in loss of its kinase activity while the K746G mutation results in enhanced kinase activity (PMID: 33459343).
      25 kDa RAB7 represents the exogenous transfected HA-RAB7, while 23 kDa RAB7 represents the endogenous RAB7 in HEK-293T.
      The identity of the bands at approximately 70 kDa is unknown.
      All the lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

    • Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Western blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      All lanes : Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (ab302494) at 1/1000 dilution

      Lane 1 : GFP-LRRK1 stably expressed HEK-293T treated with 100 ng/ml PMA for 30 minutes, whole cell lysate (untreated membrane)
      Lane 2 : GFP-LRRK1 stably expressed HEK-293T treated with 100 ng/ml PMA for 30 minutes, whole cell lysate (phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 23 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking / Diluting buffer: 5% NFDM/TBST.

      The identity of the bands at approximately 70 kDa is unknown.
      All the lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

    • Dot Blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Dot Blot - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

      Concentration of ab302494: 1/1000 dilution (0.486 μg/ml)

      Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100000 dilution

      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      Lane 1: RAB7 phospho S72 peptide a

      Lane 2: RAB7 phospho S72 peptide b

      Lane 3: RAB7 non-phospho peptide corresponding to the region covered by RAB7 pS72 -containing peptides a and b.

      Exposure time: 3 minutes

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

      Immunohistochemical analysis of paraffin-embedded human stomach labeling RAB7 (phospho S72) with ab302494 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human stomach without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab302494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

      Immunohistochemical analysis of paraffin-embedded human bladder cancer labeling RAB7 (phospho S72) with ab302494 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human bladder cancer without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab302494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)
      Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab302494 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302494 は 2 報の論文で使用されています。

    • Metcalfe RD  et al. Structure and regulation of full-length human leucine-rich repeat kinase 1. Nat Commun 14:4797 (2023). PubMed: 37558661
    • Malik AU  et al. PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain. Biochem J 479:1941-1965 (2022). PubMed: 36040231

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab302494.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com