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遺伝子ノックアウト細胞株 検証済

Anti-Rab5A 抗体 [3A4] - Early Endosome Marker (ab66746)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (13)

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Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)
  • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
  • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)

Key features and details

  • Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

こちらの製品もご検討ください

Biochemical
Product image
Wiskostatin, N-WASP inhibitor (ab141085)
タンパク質
Product image
Recombinant Human Rab5A protein (ab62956)

関連製品

製品の概要

  • 製品名

    Anti-Rab5A antibody [3A4] - Early Endosome Marker
    Rab5A 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Human Rab5a aa 1-250.

  • ポジティブ・コントロール

    • WB: Extracts of mouse and human brain. ICC/IF: U87MG cells.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    3A4
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG2b
  • 軽鎖の種類

    kappa
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Rabs
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Rab5A protein (ab62956)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab66746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在

    Cell membrane. Early endosome membrane. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable).
  • Target information above from: UniProt accession P20339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5868 Human
    • SwissProt: P20339 Human
    • Unigene: 475663 Human
    • 別名

      • RAB5A antibody
      • RAB5A_HUMAN antibody
      • Ras-related protein Rab-5A antibody

    画像

    • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
      Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hu brain whole cell lysate (20 µg)
      Lane 4: Ms brain whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

      This image was produced using the ascites version of this antibody. 

    • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)
      Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)
      All lanes : Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 lysate
      Lane 2 : RAB5A Knockout HAP1 lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      ab66746 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab66746 overnight at 4 °C at a 1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

      These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
    • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
      Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
      Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + mouse brain at 45 µg

      Secondary
      goat anti-mouse secondary antibody conjugated to HRP

      Predicted band size: 24 kDa

    • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
      Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
      Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg

      Secondary
      HRP-conjugated goat anti-mouse

      Developed using the ECL technique.

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      This image was produced using the ascites version of this antibody. 

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (13)

    ab66746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab66746 は 13 報の論文で使用されています。

    • Cao H  et al. MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling. Nat Commun 14:6190 (2023). PubMed: 37794006
    • Oh RY  et al. Biallelic loss-of-function variants in RABGAP1 cause a novel neurodevelopmental syndrome. Genet Med 24:2399-2407 (2022). PubMed: 36083289
    • Patel M  et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
    • Tang Y  et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. Acta Pharm Sin B 12:2043-2056 (2022). PubMed: 35847504
    • Muendlein HI  et al. Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses. Sci Immunol 7:eadd0665 (2022). PubMed: 36563168
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 09 2016

    Question

    That sounds great! Thank you so much. You guys are certainly my top choice for antibodies and other things going forward. Your customer service is as good as your products seem to be!

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2012

    Answer

    Thank you for your email.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab66746.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.


    Here is the information about the testing discount code:

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab66746 in ICC/IF. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICC/IFand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jul 04 2012

    Question

    He wanted me to ask if you could go ahead and send us the new antibody. We are in a bit of a time crunch and the 11 days is a bit of an issue especially if it doesn't work.

    As for which of the other two mouse antibodies to choose, I will let you pick as you have far more expertise in this area.

    Thank you so much for all your help and advice.

    What info do you need from me?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer

    Thank you for contacting us.

    Yes, I'd be happy to send you one of the mouse antibodies, but am afraid I cannot make the final decision for you. I'd be able to make a recommendation, though.
    Both antibodies (ab66746,ab55657) have not been tested inICC/IF, thus we cannot guarantee either of the antibodies forthisapplication. However, ab66746 was tested in mouse samples in WB. Thus, I would recommend using this antibody.

    Since ICC/IF is untested, I would also send you a testing discount code: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
    This programapplies to ELISA kits and other kits we offer.

    Please let me know if I shall go ahead with the free of charge replacement with ab66746 and the testing discount code for trying it in ICC/IF?

    I look forward to hear back from you and assist you further.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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