AB66746

Anti-Rab5A 抗体 [3A4] - Early Endosome Marker

Name: Anti-Rab5A 抗体 [3A4] - Early Endosome Marker (ab66746) | Abcam
Brand: Abcam Limited
SKU: AB66746
Price: 57000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Rab5A antibody [3A4] - Early Endosome Marker

KO Validated
Lab Essentials
詳細を見る

(1 Review)

(15 Publications)

Mouse Monoclonal RAB5A antibody. Early Endosome marker. Suitable for WB and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus.

別名を表示する

RAB5, RAB5A, Ras-related protein Rab-5A

4 Images

Lab

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Rab5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Hu brain whole cell lysate (20 μg)
Lane 4 : Ms brain whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

Predicted band size: 24 kDa

false

Lab

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

ab66746 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab66746 overnight at 4 °C at a 1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

RAB5A Knockout HAP1 lysate at 20 µg

Observed band size: 24 kDa

false

Unknown

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

All lanes:

extracts of mouse brain at 45 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse

Predicted band size: 24 kDa

Observed band size: 24 kDa

true

Unknown

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

All lanes:

mouse brain at 45 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 24 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

3A4

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.

Biological function summary

Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.

Pathways

Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.

Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

See full target information RAB5A

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

PLoS pathogens 20:e1012256 PubMed39024394

2024

A genome-wide CRISPR/Cas9 knockout screen identifies TMEM239 as an important host factor in facilitating African swine fever virus entry into early endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Shen,Guigen Zhang,Xiaogang Weng,Renqiang Liu,Zhiheng Liu,Xiangpeng Sheng,Yuting Zhang,Yan Liu,Yanshuang Mu,Yuanmao Zhu,Encheng Sun,Jiwen Zhang,Fang Li,Changyou Xia,Junwei Ge,Zhonghua Liu,Zhigao Bu,Dongming Zhao

Nature communications 14:6190 PubMed37794006

2023

MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

He Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai Li

Science immunology 7:eadd0665 PubMed36563168

2022

Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley I Muendlein,Wilson M Connolly,James Cameron,David Jetton,Zoie Magri,Irina Smirnova,Edouard Vannier,Xudong Li,Amanda J Martinot,Rebecca Batorsky,Alexander Poltorak

Genetics in medicine : official journal of the American College of Medical Genetics 24:2399-2407 PubMed36083289

2022

Biallelic loss-of-function variants in RABGAP1 cause a novel neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Youjin Oh,Ashish R Deshwar,Ashish Marwaha,Nesrin Sabha,Michael Tropak,Huayun Hou,Kyoko E Yuki,Michael D Wilson,Patrick Rump,Roelineke Lunsing,Noha Elserafy,Clara W T Chung,Stacy Hewson,Tanja Klein-Rodewald,Julia Calzada-Wack,Adrián Sanz-Moreno,Markus Kraiger,Susan Marschall,Helmut Fuchs,Valerie Gailus-Durner,Martin Hrabe de Angelis,James Dowling,Andreas Schulze

Acta pharmaceutica Sinica. B 12:2043-2056 PubMed35847504

2022

The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyun Tang,Jinchao Gao,Tao Wang,Qian Zhang,Antian Wang,Meng Huang,Renhe Yu,Hongzhuan Chen,Xiaoling Gao

Frontiers in immunology 13:833515 PubMed35309340

2022

Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lam Ng,Xiaohui Wang,Chuanbin Yang,Chengfu Su,Min Li,Allen Ka Loon Cheung

British journal of cancer 126:1704-1714 PubMed35173303

2022

Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meera Patel,Kathryn A F Pennel,Jean A Quinn,Hannah Hood,David K Chang,Andrew V Biankin,Selma Rebus,Antonia K Roseweir,James H Park,Paul G Horgan,Donald C McMillan,Joanne Edwards

PLoS genetics 17:e1009551 PubMed34714826

2021

Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Huai-Wei Huang,Hyung Don Ryoo

Cell reports 35:109168 PubMed34038728

2021

Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Aikaterini Nanou,Mara Bourbouli,Stefania Vetrano,Ute Schaeper,Steven Ley,George Kollias

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab5A 抗体 [3A4] - Early Endosome Marker

Anti-Rab5A antibody [3A4] - Early Endosome Marker

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (15)

Abcam product promise