AB18211

Anti-Rab5 抗体 - Early Endosome Marker

Name: Anti-Rab5 抗体 - Early Endosome Marker (ab18211) | Abcam
Brand: Abcam Limited
SKU: AB18211
Rating: 4 (26 reviews)

Anti-Rab5 antibody - Early Endosome Marker

Lab Essentials
詳細を見る

(26 Reviews)

(135 Publications)

Anti-Rab5 antibody - Early Endosome Marker (ab18211) is a rabbit polyclonal antibody detecting Rab5 in Western Blot. Suitable for Human, Mouse, Rat.

- Over 110 publications
- Trusted since 2005

別名を表示する

RAB5, RAB5A, Ras-related protein Rab-5A

4 Images

Lab

Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

ab18211 staining Rab5 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18211 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Project911****

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

All lanes:

Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate (ab27253) at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 8:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Project911****

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

All lanes:

Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/mL

All lanes:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

Predicted band size: 24 kDa,47 kDa

Observed band size: 24 kDa,40 kDa,67 kDa,70 kDa

false

Unknown

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

All lanes:

Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Rab5A protein (<a href='/products/proteins-peptides/recombinant-human-rab5a-protein-ab62956'>ab62956</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

true

Exposure time: 150s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The immunogen used for this product shares 43% homology with Rab5C. Cross-reactivity with this protein has not been confirmed experimentally.
Replenishment batches of our polyclonal antibody, ab18211 are tested in WB. Previous batches were additionally validated in ICC. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab218624.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-5 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Rab5 antibody - Early Endosome Marker (ab18211) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of Rab5?
Anti-Rab5 - Early Endosome Marker (ab18211) specifically detects a band for Rab5 (UniProt: P20339) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-Rab5 - Early Endosome Marker (ab18211) was first used in a scientific publication in 2005 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-Rab5 - Early Endosome Marker (ab18211) has over 25 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.

Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

See full target information RAB5A

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文献 (135)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113062 PubMed40740492

2025

FMRP-dependent translational control negatively regulates adapter protein complex 2-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Shi,Adam Kosti,Nisha Raj,Jiarong Sun,Yuhao Gao,Roy Simamora,Arielle N Valdez-Sinon,Victor Faundez,Gary J Bassell

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70609 PubMed40525396

2025

LSMEM2, Localized at the Neuromuscular Junction, Modulates Mitochondrial Integration in Skeletal Muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Elrefaei,Satoru Yamazaki,Issei Yazawa,Yusuke Takahashi,Naoki Ito,Nozomi Hayashiji,Yuya Nishida,Ichizo Nishino,Seiji Takashima,Yasunori Shintani

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed40514243

2025

Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer's Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Jiang,Kuldeep Sachdeva,Chris N Goulbourne,Martin J Berg,James Peddy,Philip H Stavrides,Anna Pensalfini,Monika Pawlik,Sandeep Malampati,Lauren Whyte,Balapal S Basavarajappa,Shivakumar Subbanna,Cynthia Bleiwas,John F Smiley,Paul M Mathews,Ralph A Nixon

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

Cell death & disease 16:328 PubMed40263294

2025

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Rasika Rathnayake,Jia Zeng,Jing Huang,Gary Iacobucci,Michael C Yu,Shermali Gunawardena

Cellular and molecular life sciences : CMLS 82:128 PubMed40128454

2025

The HBV large envelope protein initiates virion assembly by recruiting capsids at membrane rich domains related to late endosome.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Seigneuret,Sébastien Eymieux,Vanessa Sarabia-Vega,Roxane Lemoine,Julien Burlaud-Gaillard,Pierre Raynal,Christophe Hourioux,Camille Sureau,Philippe Roingeard,Hugues de Rocquigny

Translational neurodegeneration 13:54 PubMed39497162

2024

Critical role of ROCK1 in AD pathogenesis via controlling lysosomal biogenesis and acidification.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghuan Song,Wanying Huang,Pingao Zhang,Jiyun Shi,Ting Yu,Jing Wang,Yongbo Hu,Lanxue Zhao,Rui Zhang,Gang Wang,Yongfang Zhang,Hongzhuan Chen,Hao Wang

International journal of molecular sciences 25: PubMed39408688

2024

Effect of CFTR Modulators on Oxidative Stress and Autophagy in Non-CFTR-Expressing Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Filippo Scialò,Gustavo Cernera,Lorenza Polise,Giuseppe Castaldo,Felice Amato,Valeria Rachela Villella

Cellular and molecular life sciences : CMLS 81:416 PubMed39367928

2024

Apache is a neuronal player in autophagy required for retrograde axonal transport of autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Parisi,Alessandro Esposito,Enrico Castroflorio,Mattia Bramini,Sara Pepe,Antonella Marte,Fabrizia C Guarnieri,Flavia Valtorta,Pietro Baldelli,Fabio Benfenati,Anna Fassio,Silvia Giovedì

Folia histochemica et cytobiologica 62:123-132 PubMed39329500

2024

Changes in early endosomes in rat hippocampal CA1 neurons after transient global cerebral ischaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Min Qiang,Bai-Hong Tan,De-Sheng Huo,Shu-Lei Li,Zi-Zhen Fan,Ze-Qun Zhou,Rong-Yu Wang,Yanchao Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab5 抗体 - Early Endosome Marker

Anti-Rab5 antibody - Early Endosome Marker

Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

Western blot - Anti-Rab5 antibody - Early Endosome Marker (AB18211)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (135)

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