Anti-Rab5 抗体 - Drosophila Early Endosome Marker (ab31261)
Key features and details
- Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Rab5 antibody - Drosophila Early Endosome Marker
Rab5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
Immunogen の所有権に関して (Peptide available as ab32489.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31261の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Required for the fusion of plasma membranes and early endosomes. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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別名
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
画像
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Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (68)
ab31261 は 68 報の論文で使用されています。
- O'Brien CE et al. The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling. J Cell Biol 222:N/A (2023). PubMed: 36239632
- Nithianandam V et al. Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging. Nat Commun 14:7034 (2023). PubMed: 37923712
- Boda A et al. Rab26 controls secretory granule maturation and breakdown in Drosophila. Cell Mol Life Sci 80:24 (2023). PubMed: 36600084
- Song C & Broadie K Fragile X mental retardation protein coordinates neuron-to-glia communication for clearance of developmentally transient brain neurons. Proc Natl Acad Sci U S A 120:e2216887120 (2023). PubMed: 36920921
- Wang L et al. Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in glia. Sci Adv 8:eabn3326 (2022). PubMed: 35857503