Anti-Rab5 抗体 (ab13253)
Key features and details
- Rabbit polyclonal to Rab5
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Rab5 antibody
Rab5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rab5 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human Rab5 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: P20339 -
ポジティブ・コントロール
- ICC/IF: Rat hippocampal neurons (embryonic day 18), HEK cells, HeLa cells. IHC-P: Mouse back skin tissue section.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13253の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100.
Use Bouin's fixative to fix tissue. |
|
ICC/IF |
1/80.
|
特記事項 |
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IHC-P
1/100. Use Bouin's fixative to fix tissue. |
ICC/IF
1/80. |
ターゲット情報
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機能
Required for the fusion of plasma membranes and early endosomes. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 404008 Dog
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P18066 Dog
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
see all -
別名
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceConfocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
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Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Human cervical cancer cell line (HeLa) for 20min at RT, labelling Rab5 with ab13253 at 1/80 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody (green). (C) Composite image.
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ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab13253 は 32 報の論文で使用されています。
- Bagalkot TR et al. Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments. J Neurosci 41:234-250 (2021). PubMed: 33234607
- Kusama K et al. Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions. Front Genet 12:676791 (2021). PubMed: 34262596
- Han J et al. Endocytic BDNF secretion regulated by Vamp3 in astrocytes. Sci Rep 11:21203 (2021). PubMed: 34707216
- Puangmalai N et al. Internalization mechanisms of brain-derived tau oligomers from patients with Alzheimer's disease, progressive supranuclear palsy and dementia with Lewy bodies. Cell Death Dis 11:314 (2020). PubMed: 32366836
- Carim SC et al. IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis. Curr Biol 29:775-789.e7 (2019). PubMed: 30799246