AB194827

Anti-RAB45 抗体 [EPR16349]

Anti-RAB45 antibody [EPR16349]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RAB45 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

別名を表示する

RAB45, RASEF, Ras and EF-hand domain-containing protein, Ras-related protein Rab-45

5 Images

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (AB194827)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (AB194827)

Intracellular Flow Cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody : Blue. Rabbit monoclonal IgG was used as isotype control (Black).

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (AB194827)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (AB194827)

Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm staining on HeLa cell line was observed.

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,83 kDa

false

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

RAW 264.7 cell lysate at 10 µg

Lane 8:

PC-12 cell lysate at 10 µg

Lane 9:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16349

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RAB45 also known as RASEF is a member of the Ras superfamily of small GTPases. This protein has a molecular mass of approximately 83 kDa. It regulates various cellular processes by cycling between inactive GDP-bound and active GTP-bound states. RAB45 primarily localizes to intracellular membranes including endosomes and lysosomes. It is highly expressed in tissues such as the brain and pancreas indicating its role in specific cellular functions.

Biological function summary

RAB45 participates in intracellular trafficking ensuring proper vesicle flow and cargo delivery in the cell. It may associate with various effector proteins forming complexes needed for its function. Its role in membrane trafficking links it to pathways involving cellular signaling and homeostasis. RAB45 helps maintain cellular organization by managing membrane-bound compartments which is important for the effective function of endosomal and lysosomal pathways.

Pathways

RAB45 plays roles in vesicular transport and endocytic recycling processes. It interacts within the Rab family pathways that facilitate membrane trafficking. RAB45 is closely related to other Rab family proteins such as RAB7 and RAB9 which also regulate endosomal and lysosomal pathways. These interactions ensure coordination of intracellular transport and recycling processes necessary for cellular maintenance and function.

RAB45 shows associations with certain neurological disorders and cancer types. Altered expression levels of RAB45 have been reported in glioblastoma suggesting its potential role in tumor progression. Additionally RAB45 might influence Alzheimer's disease by impacting vesicular pathways tied to APP processing. Connections with proteins such as tau in neurodegenerative pathways highlight the possible implications of RAB45 dysregulation in disease states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds predominantly GDP, and also GTP (PubMed : 17448446). Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules (PubMed : 30814157).

See full target information RASEF

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:3307-3324.e30 PubMed37385249

2023

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Joleen S Cheah,Charles Xu,James Messing,Brian D Freibaum,Steven Boeynaems,J Paul Taylor,Namrata D Udeshi,Steven A Carr,Alice Y Ting

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB45 抗体 [EPR16349]

Anti-RAB45 antibody [EPR16349]

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (AB194827)

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (AB194827)

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

Western blot - Anti-RAB45 antibody [EPR16349] (AB194827)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise