Anti-Rab4 抗体 - Early Endosome Marker (ab13252)
Key features and details
- Rabbit polyclonal to Rab4 - Early Endosome Marker
- Suitable for: WB, ICC
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Rab4 antibody - Early Endosome Marker
Rab4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rab4 - Early Endosome Marker -
由来種
Rabbit -
特異性
This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat -
免疫原
The details of the immunogen for this antibody are not available.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
|
ICC | (1) |
1/200 - 1/500.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa). |
ICC
1/200 - 1/500. |
ターゲット情報
-
機能
Protein transport. Probably involved in vesicular traffic. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
翻訳後修飾
Phosphorylated by CDK1 kinase during mitosis. -
細胞内局在
Membrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1. - Information by UniProt
-
参照データベース
- Entrez Gene: 25532 Rat
- SwissProt: P05714 Rat
- Unigene: 3016 Rat
-
別名
- HRES 1 / RAB4 antibody
- Oncogene RAB4 antibody
- Rab 4 antibody
see all
画像
-
Immunocytochemistry - Anti-Rab4 antibody - Early Endosome Marker (ab13252)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
ab13252, rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-beta-tubulin II at a 1/400 dilution, followed by secondary goat anti-mouse Alexa Fluor 568.
-
Anti-Rab4 antibody - Early Endosome Marker (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue
Predicted band size: 25.6 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (37)
ab13252 は 37 報の論文で使用されています。
- Giridharan SSP et al. Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. Elife 11:N/A (2022). PubMed: 35040777
- Rogers MA et al. PDGFR dimer-specific activation, trafficking and downstream signaling dynamics. J Cell Sci 135:N/A (2022). PubMed: 35946433
- Pazzaglia S et al. Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis. Int J Mol Sci 22:N/A (2021). PubMed: 33924260
- Actis Dato V & Chiabrando GA Activated Alpha-2 Macroglobulin Improves Insulin Response via LRP1 in Lipid-Loaded HL-1 Cardiomyocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34203120
- Souza ILM et al. Endocytosis of the non-catalytic ADAM23: Recycling and long half-life properties. Exp Cell Res 398:112415 (2021). PubMed: 33296662