Anti-RAB3GAP2 抗体 (ab110462)
Key features and details
- Rabbit polyclonal to RAB3GAP2
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-RAB3GAP2 antibody -
製品の詳細
Rabbit polyclonal to RAB3GAP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide derived from an internal region of Human RAB3GAP2 (UniProt ID: Q9H2M9).
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ポジティブ･コントロール
- Human breast carcinoma tissue; extracts from COS cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab110462 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110462の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa).
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| IHC-P |
1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa). |
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IHC-P
1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes. |
ターゲット情報
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関連性
RAB3GAP2 is a regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. It is required for normal eye and brain development and may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. -
細胞内局在
Cytoplasmic. Note: In neurons, it is enriched in the synaptic soluble fraction. -
参照データベース
- Entrez Gene: 25782 Human
- Entrez Gene: 98732 Mouse
- Entrez Gene: 289350 Rat
- Omim: 609275 Human
- SwissProt: Q9H2M9 Human
- SwissProt: Q8BMG7 Mouse
- SwissProt: Q5U1Z0 Rat
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別名
- DKFZp434D245 antibody
- FLJ14579 antibody
- KIAA0839 antibody
see all
画像
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Western blot - Anti-RAB3GAP2 antibody (ab110462)All lanes : Anti-RAB3GAP2 antibody (ab110462) at 1/500 dilution
Lane 1 : Extracts from COS cells
Lane 2 : Extracts from COS cells, treated with the synthesized peptide
Lysates/proteins at 30 µg per lane.
Predicted band size: 156 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB3GAP2 antibody (ab110462)ab110462, at a 1/50 dilution, staining RAB3GAP2 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110462 は論文での使用が確認できていません。