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リコンビナントRabMAb

Anti-RAB3B 抗体 [EPR12987] (ab177949)

Reviews (1) Submit a question
Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (ab177949)
  • Western blot - Anti-RAB3B antibody [EPR12987] (ab177949)
  • Anti-RAB3B antibody [EPR12987] (ab177949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12987] to RAB3B
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RAB3B antibody [EPR12987]
    RAB3B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12987] to RAB3B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T, HepG2, fetal brain and SW480 lysates; SW480 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177949の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

画像

  • Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (ab177949)
    Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (ab177949)

    Intracellular flow cytometric analysis of permeabilized SW480 cellslabeling RAB3B with ab177949at 1/10 dilution (red) compared to a rabbit IgG negative control (green). 

     

     

  • Western blot - Anti-RAB3B antibody [EPR12987] (ab177949)
    Western blot - Anti-RAB3B antibody [EPR12987] (ab177949)
    All lanes : Anti-RAB3B antibody [EPR12987] (ab177949) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : SW480 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Anti-RAB3B antibody [EPR12987] (ab177949)
    Anti-RAB3B antibody [EPR12987] (ab177949)

参考文献 (0)

ab177949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab177949 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB3B antibody [EPR12987]

 Average
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Oct 11 2018

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