Anti-Rab3A 抗体 (ab3335)
Key features and details
- Rabbit polyclonal to Rab3A
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rab3A antibody
Rab3A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rab3A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Rab3A aa 1-18.
Sequence:MASATDSRYGQKESSDQN
(Peptide available asab4951) -
ポジティブ･コントロール
- WB: mouse brain, kidney, liver, rat brain, kidney, liver; ICC: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Protein A purified -
一次抗体 備考
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3335の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (7) |
Use a concentration of 2 µg/ml.
|
| ICC/IF | (1) |
Use a concentration of 2 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. |
|
ICC/IF
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. -
組織特異性
Specifically expressed in brain. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5864 Human
- Entrez Gene: 19339 Mouse
- Entrez Gene: 25531 Rat
- Omim: 179490 Human
- SwissProt: P20336 Human
- SwissProt: P63011 Mouse
- SwissProt: P63012 Rat
- Unigene: 27744 Human
see all -
別名
- Rab 3A antibody
- RAB 3A member RAS oncogene family antibody
- Rab3a antibody
see all
画像
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Western blot - Anti-Rab3A antibody (ab3335)All lanes : Left: ab3335 at 2 µg/mL.
Right: ab302518 at 1/1000 dilution
Lane 1 : Wild-type SK-N-FI cell lysate
Lane 2 : RAB3A knockout SK-N-FI cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 27 kDa why is the actual band size different from the predicted?Western blot: Left: Anti-RAB3A antibody (ab3335) staining at 2 ug/ml. Right: Recombinant Anti-Rab3A antibody [EPR26022-9] (ab302518) staining at 1/1000 dilution. Anti-Rab3A antibodies shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab3335 and ab302518 were shown to bind specifically to RAB3A. A band was observed at 27 kDa in wild-type SK-N-FI cell lysates with no signal observed at this size in RAB3A knockout cell line. To generate this image, wild-type and RAB3A knockout SK-N-FI cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab3335)Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa (Human epithelial adenocarcinoma cell line)
cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3335 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. -
Western blot - Anti-Rab3A antibody (ab3335)All lanes : Anti-Rab3A antibody (ab3335) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat kidney tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilutionA 27 kDa band corresponding to RAB3A was observed along with two uncharacterized band (*) at ~40 kD and 60 kDa across tissues tested.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab3335 は 17 報の論文で使用されています。
- Astillero-Lopez V et al. Neurodegeneration and astrogliosis in the entorhinal cortex in Alzheimer's disease: Stereological layer-specific assessment and proteomic analysis. Alzheimers Dement 18:2468-2480 (2022). PubMed: 35142030
- Du K et al. Rapid isolation and cryo-EM characterization of synaptic vesicles from mammalian brain. FEBS Open Bio 12:1980-1987 (2022). PubMed: 36000326
- Prasai B et al. The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Nat Commun 12:3970 (2021). PubMed: 34172739
- Wauters F et al. LRRK2 mutations impair depolarization-induced mitophagy through inhibition of mitochondrial accumulation of RAB10. Autophagy 16:203-222 (2020). PubMed: 30945962
- Connor-Robson N et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2. Neurobiol Dis 127:512-526 (2019). PubMed: 30954703