Anti-RAB35 抗体 [EPR24487-21] (BSA and Azide free) (ab300117)
![Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)](https://www.abcam.co.jp/ps/products/300/ab300117/Images/AB300117-485981-WB2.jpg "Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24487-21] to RAB35 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free)
RAB35 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24487-21] to RAB35 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Rat and mouse brain, Caco-2, 293T, C6, RAW264.7, PC-12, NIH/3T3, RAB35 full length protein lysates.\ IP: Caco-2 cell.
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特記事項
Ab300117 is a carrier free version of ab300116.
Ab300116 does not reactin in IP with Mouse species.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24487-21 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300117の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Endosome. Melanosome. Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 11021 Human
- Entrez Gene: 77407 Mouse
- Entrez Gene: 288700 Rat
- Omim: 604199 Human
- SwissProt: Q15286 Human
- SwissProt: Q6PHN9 Mouse
- SwissProt: Q5U316 Rat
- Unigene: 524788 Human
see all -
別名
- GTP binding protein RAY antibody
- GTP-binding protein RAY antibody
- H ray antibody
see all
画像
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Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)All lanes : Anti-RAB35 antibody [EPR24487-21] (ab300116) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab300116, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29662076; PMID: 34349123 ).
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![Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)](https://www.abcam.co.jp/ps/products/300/ab300117/Images/AB300117-485978-WB1.jpg)
Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)All lanes : Anti-RAB35 antibody [EPR24487-21] (ab300116) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab300116, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23572513 ).
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![Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)](https://www.abcam.co.jp/ps/products/300/ab300117/Images/AB300117-485977-WB3.jpg)
Western blot - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)All lanes : Anti-RAB35 antibody [EPR24487-21] (ab300116) at 1/1000 dilution
Lane 1 : RAB35 full-length protein 10 ng
Lane 2 : RAB family protein homologous region 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsThis data was developed using ab300116, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This antibody has no cross-reactivity with other RAB family proteins.
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![Immunoprecipitation - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)](https://www.abcam.co.jp/ps/products/300/ab300117/Images/AB300117-2-anti-rab35-antibody-epr2448721-immunoprecipitation-caco2.jpg)
Immunoprecipitation - Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (AB300117)This data was developed using ab300116, the same antibody clone in a different buffer formulation.
RAB35 was immunoprecipitated from 0.35 mg Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab300116 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300116 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 (Input): Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): Caco-2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300116 in Caco-2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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![Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (ab300117)](https://www.abcam.co.jp/ps/products/300/ab300117/Images/AB300117-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-RAB35 antibody [EPR24487-21] (BSA and Azide free) (ab300117)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300117 は論文での使用が確認できていません。
