Anti-RAB29 (phospho S72) 抗体 [MJF-R40-R3-H3] (ab291075)
![Western blot - Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)](https://www.abcam.co.jp/ps/products/291/ab291075/Images/ab291075-485584-MJF40WB1.jpg "Western blot - Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R40-R3-H3] to RAB29 (phospho S72)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3]
RAB29 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [MJF-R40-R3-H3] to RAB29 (phospho S72) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates of HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing a HA-tag. RAB29 expression vector contained either the single T71A mutation or both T71A and S72A mutations. Cells were either treated or not treated with 200 µM MLi-2 for 1.5h. All variations were HA-tagged.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed with support from The Michael J. Fox Foundation.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MJF-R40-R3-H3 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab291075の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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| 特記事項 |
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WB
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
ターゲット情報
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機能
Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
翻訳後修飾
In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847). -
細胞内局在
Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery. - Information by UniProt
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参照データベース
- Entrez Gene: 8934 Human
- Omim: 603949 Human
- SwissProt: O14966 Human
- Unigene: 115325 Human
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別名
- DKFZp686P1051 antibody
- Rab 7 like protein 1 antibody
- RAB 7L antibody
see all
画像
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Western blot - Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)All lanes : Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075) at 1/500 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing an HA-tag, whole cell lysate
Lane 2 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate,
Lane 3 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A mutation) expression vector containing an HA-tag, whole cell lysate.
Lane 4 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lane 5 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (S72A mutation) expression vector containing an HA-tag, whole cell lysate
Lane 6 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (S72A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lane 7 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A and S72A mutation) expression vector containing an HA-tag, whole cell lysate
Lane 8 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A and S72A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lysates/proteins at 38 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
All the lysates were kindly provided by Prof. Dario Alessi, University of Dundee.
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![Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)](https://www.abcam.co.jp/ps/products/291/ab291075/Images/ab291075-485590-Benefits-of-Recombinant-antibodies.jpg)
Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab291075 は論文での使用が確認できていません。