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リコンビナントRabMAb

Anti-RAB29 (phospho S72) 抗体 [EPR25173-80] - BSA and Azide free (ab302703)

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Western blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
  • Immunoprecipitation - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
  • Dot Blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
  • Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25173-80] to RAB29 (phospho S72) - BSA and Azide free
  • Suitable for: IP, WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-RAB29 (phospho S72) antibody [EPR25173-80] - BSA and Azide free
    RAB29 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25173-80] to RAB29 (phospho S72) - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, Dot blotmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate IP: HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag. Dot Blot: RAB29 (phospho S72) peptide.

  • 特記事項

    AB302703 is a carrier free version of AB302702. 

    AB302702 does not react in: IHC-P with human, mouse, and rat samples; ICC/IF and intracellular flow cytmometry with human samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302703の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 20-30 kDa (predicted molecular weight: 23 kDa).
Dot blot
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 20-30 kDa (predicted molecular weight: 23 kDa).
Dot blot
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 翻訳後修飾

    In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).

  • 細胞内局在

    Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp686P1051 antibody
      • Rab 7 like protein 1 antibody
      • RAB 7L antibody
      • Rab-7-like protein 1 antibody
      • RAB29 antibody
      • Rab7 like protein 1 antibody
      • RAB7 member RAS oncogene family like 1 antibody
      • RAB7L antibody
      • RAB7L_HUMAN antibody
      • Ras related protein Rab 7L1 antibody
      • Ras related protein Rab7L1 antibody
      • Ras-related protein Rab-29 antibody
      • Ras-related protein Rab-7L1 antibody
      see all

    画像

    • Western blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
      Western blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
      All lanes : Anti-RAB29 (phospho S72) antibody [EPR25173-80] (ab302702) at 1/1000 dilution

      Lane 1 : HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate
      Lane 2 : HEK-293T cellls co-transfected with a human RAB29 (S72A mutant) expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate
      Lane 3 : HEK-293T cells transfected with a human RAB29 expression vector containing a Myc-his tag, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 23 kDa
      Observed band size: 20-30 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302702, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST. 

      Rab29-S72 is phosphorylated by LRRK2 (PMID: 29125462).
      Two bands detect in OE cell was due to endogenous expression Rab29 in HEK293 and overexpression Rab29(PMID: 33135724). 

    • Immunoprecipitation - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
      Immunoprecipitation - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)

      This data was developed using ab302702, the same antibody clone in a different buffer formulation. 

      RAB29 (phospho S72) was immunoprecipitated from 0.35 mg HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag whole cell lysate with AB302702 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using AB302702 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate 10 µg (Inset)

      Lane 2: ab302702 IP in HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302702 in HEK-293T cells co-transfected with a human RAB29 expression vector containing a Myc-his tag and a human LRRK2 (Y1699C mutant) expression vector containing a flag tag, whole cell lysate

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 67 seconds. 

      Observed MW (kDa): 20-30.

    • Dot Blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
      Dot Blot - Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)

      This data was developed using ab302702, the same antibody clone in a different buffer formulation. 

      Dot blot analysis of RAB29 (phospho S72) using ab302702 at 1/1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

      Lane 1: RAB29 (phospho S72) peptide
      Lane 2: RAB29 non-phospho peptide
      Lane 3: RAB29 (phospho S72/T71) peptide
      Lane 4: RAB29 (phospho T71) peptide

      Exposure time: 180 seconds.

      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)
      Anti-RAB29 (phospho S72) antibody [EPR25173-80] (BSA and Azide free) (AB302703)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302703 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302703 は論文での使用が確認できていません。

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