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  1. Link

    products/primary-antibodies/rab27a-antibody-1g7-ab55667.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-RAB27A 抗体 [1G7] (ab55667)

  • Datasheet
Reviews (10)Q&A (3)References (46)

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Western blot - Anti-RAB27A antibody [1G7] (ab55667)
  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody [1G7] (ab55667)
  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)

Key features and details

  • Mouse monoclonal [1G7] to RAB27A + RAB27B
  • Suitable for: WB, IHC-P, ICC/IF, IP, IHC-FoFr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

こちらの製品もご検討ください

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関連製品

製品の概要

  • 製品名

    Anti-RAB27A antibody [1G7]
  • 製品の詳細

    Mouse monoclonal [1G7] to RAB27A + RAB27B
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IP, IHC-FoFrmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Recombinant fragment corresponding to Human RAB27A aa 122-221.
    Sequence:

    YCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNIS QAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC


    Database link: P51159
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: HAP1, Jurkat, MCF7 whole cell lysate; HL-60 cells; 293 cells, IHC-P: Human lymphoma. ICC/IF: Cow Retinal Pigment Epithelium (RPE) cells
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.4
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    1G7
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab55667の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P (2)
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
IP (1)
Use at an assay dependent concentration.

See Abreview.

IHC-FoFr
Use at an assay dependent concentration. PubMed: 21832089
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

See Abreview.

IHC-FoFr
Use at an assay dependent concentration. PubMed: 21832089

ターゲット情報

  • 細胞内局在

    RAB27A: Membrane. Melanosome. Late endosome. Lysosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles. RAB27B: Membrane.
  • 参照データベース

    • Entrez Gene: 5873 Human
    • Entrez Gene: 5874 Human
    • Entrez Gene: 11891 Mouse
    • Entrez Gene: 80718 Mouse
    • Entrez Gene: 50645 Rat
    • Entrez Gene: 84590 Rat
    • Omim: 603868 Human
    • Omim: 603869 Human
    • SwissProt: O00194 Human
    • SwissProt: P51159 Human
    • SwissProt: Q99P58 Mouse
    • SwissProt: Q9ERI2 Mouse
    • SwissProt: P23640 Rat
    • SwissProt: Q99P74 Rat
    • Unigene: 25318 Human
    • Unigene: 654978 Human
    • Unigene: 246753 Mouse
    • Unigene: 480676 Mouse
    • Unigene: 112603 Rat
    • Unigene: 37360 Rat
    see all

画像

  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Western blot - Anti-RAB27A antibody [1G7] (ab55667)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: RAB27A knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab55667 observed at 27 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab55667 was shown to specifically react with RAB27A when RAB27A knockout samples were used. Wild-type and RAB27A knockout samples were subjected to SDS-PAGE. Ab55667 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2.5 ug/ml and 1/10000 dilution respectively. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Anti-RAB27A antibody [1G7] (ab55667) + HL-60 cell lysate

    Predicted band size: 25 kDa



    This image was generated using the ascites version of the product.

  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    All lanes : Anti-RAB27A antibody [1G7] (ab55667)

    Lane 1 : RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi
    Lane 2 : Non-transfected control

    Predicted band size: 25 kDa



    GAPDH (36.1 kDa) used as specificity and loading control.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    All lanes : Anti-RAB27A antibody [1G7] (ab55667)

    Lane 1 : RAB27A transfected lysate(24.9 KDa)
    Lane 2 : Non-transfected lysate

    Predicted band size: 25 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody [1G7] (ab55667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody [1G7] (ab55667)

    Anti-RAB27A + RAB27B antibody [1G7] (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RAB27A antibody [1G7] (ab55667)
    Western blot - Anti-RAB27A antibody [1G7] (ab55667)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
    All lanes : Anti-RAB27A antibody [1G7] (ab55667) at 1/200 dilution

    Lane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vector
    Lane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-mouse

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 30 minutes


    Rab27a (25kDa)+GFP(27kDa)=52kDa

    The blot was blocked with 5% milk for 30 minutes at 25°C and incubated with the primary antibody for 12 hours at 4°C.

    This image was generated using the ascites version of the product.

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (46)

ab55667 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab55667 は 46 報の論文で使用されています。

  • Wang H  et al. Expression and significant roles of the lncRNA NEAT1/miR-493-5p/Rab27A axis in ulcerative colitis. Immun Inflamm Dis 11:e814 (2023). PubMed: 37249278
  • Park YJ  et al. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics 13:3914-3924 (2023). PubMed: 37554281
  • Gao R  et al. Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway. Front Pharmacol 14:1081030 (2023). PubMed: 36814484
  • Shin JM  et al. Sulfisoxazole Elicits Robust Antitumour Immune Response Along with Immune Checkpoint Therapy by Inhibiting Exosomal PD-L1. Adv Sci (Weinh) 9:e2103245 (2022). PubMed: 34927389
  • Saliba J  et al. Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironment. Oncogene 41:1563-1575 (2022). PubMed: 35091681
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 13 Abreviews or Q&A

Western blot abreview for Anti-RAB27A antibody [1G7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 04 2021

Western blot abreview for Anti-RAB27A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 09 2018

Western blot abreview for Anti-RAB27A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Dermal Microvascular Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
Human Dermal Microvascular Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 23 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RAB27A antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Blocking Solution Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 01 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RAB27A antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 20 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB27A antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SK-MEL-28)
Specification
SK-MEL-28
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X in PBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 18 2012

Immunoprecipitation abreview for Anti-RAB27A antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with hRab27a-GFP)
Total protein in input
20 µg
Specification
HEK 293 cells transfected with hRab27a-GFP
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

投稿 Feb 29 2008

Question

Bonjour,
Je vous envoie le questionnaire concernant l’anticorps ab55667 anti-Rab27a  ainsi que deux images du marquage obtenu.
Merci d’avance pour votre aide.
Bonne journée.

Read More

Abcam community

Verified customer

Asked on Jul 03 2012

Answer

Selon nos informations vous avez connu quelques difficultés avec ab55667 et avez contacté notre service scientifique (en février).

Nous vous avons donné quelques suggestions pour améliorer le protocole. Je vous écris maintenant pour savoir si ces suggestions ont permis de résoudre le problème ou si nous pouvions vous aider autrement? J'aimerais bien aussi vous rappeler que nous avons une garantie sur nos produits, et si nous arrivons donc pas à résoudre le problème, nous allons vous le remplacer.

Je vous souhaite bonne chance dans vos recherches. N’hésitez pas à me recontacter pour plus d’assistance.

Read More

Abcam Scientific Support

Answered on Jul 03 2012

Question

Bonjour,

Je vous envoie le questionnaire concernant l'anticorps ab55667 anti-Rab27a ainsi que deux images du marquage obtenu.

Merci däavance pour votre aide.

Bonne journee.

Read More

Abcam community

Verified customer

Asked on Feb 21 2012

Answer

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée d'apprendre que vous avez des difficultés à obtenir de bons résultats avec ab55667.

Merci d'avoir patienté pendant que je discutais avec le laboratoire. Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 180 jours, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir en compensation.

J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. J'apprécierais si vous pouviez confirmer quelques détails de la procédure.

1.)Effectivement, je pense qu'il est possible que vous voyez que du bruit de fond. Les anticorps ont tendance à précipiter sur le noyau. Donc à trop forte concentration, il se peux que ce donne un bruit de fond nucléaire. Je peux donc vous conseiller de diluer l'anticorps à 1/200 et 1/400.

2.) Pour obtenir un marquage positive, je vous conseille de utiliser du démasquage à la chaleur avec un tampon Citrate. Effectivement, le laboratoire utilise cet méthode pour détecter Rab27a avec cet anticorps.

J'espère que ces informations vous aideront. Veuillez s'il vous plaît me tenir au courant de comment vous procédez. Veuillez ne pas hésiter non plus à nous recontacter si vous avez des questions.

Read More

Abcam Scientific Support

Answered on Feb 21 2012

Question

phone call: High background on human skin paraffin sections.

Read More

Abcam community

Verified customer

Asked on Feb 16 2012

Answer

Merci pour votre appel. Je suis désolée d'apprendre que ab55667 ne vous donne pas de bons résultats en immunohistochimie.

Veuillez trouver ci-joint un questionnaire, que nous avons discuté, qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps. Veuillez s'il vous plaît nous envoyer aussi une image avec les résultats obtenus.
Ceci-ci nous permettra de vous fournir la meilleure assistance technique.

Si nous concluons que notre produit est fautif et ne fonctionne pas comme explicité par notre fiche technique, nous serons ravis de mettre en place l'envoi d'un tube de remplacement gratuit ou d'un remboursement (sous réserve d'une commande ultérieure de 180 jours).

Je vous remercie par avance de nous envoyer votre protocole.

Read More

Abcam Scientific Support

Answered on Feb 16 2012

1-10 of 13 Abreviews or Q&A

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