Anti-Rab1A 抗体 [EPR27169-83] (ab302545)

製品名

Anti-Rab1A antibody [EPR27169-83]
Rab1A 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR27169-83] to Rab1A
由来種

Rabbit
アプリケーション

適用あり: ICC/IF, IP, IHC-P, Flow Cyt (Intra), WB, Dot blotmore details
適用なし: IHC-Fr
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Whole cell lysates: U-87 MG, U-2 OS, A549, HCT116, NIH/3T3, C2C12, C6, human stomach, mouse and rat brain and liver, HAP1 lysate. IHC-P: Human stomach, mouse and rat kidney. ICC/IF: A549, C2C12. Flow cyt. Intr.: A549, C2C12. IP: A549, C2C12. DB: Human Ras-related protein Rab-1A immunogen peptide.
特記事項
ab302545 does not react in IHC-Frozen with mouse and rat species.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR27169-83
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

Alternative Versions
- Anti-Rab1A antibody [EPR27169-83] (BSA and Azide free) (ab302546)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab302545の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF		1/500.
IP		1/30.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)		1/50.
WB		1/200 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Dot blot		1/1000.

特記事項
ICC/IF 1/500.
IP 1/30.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra) 1/50.
WB 1/200 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Dot blot 1/1000.

追加情報

Is unsuitable for IHC-Fr.

機能

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
配列類似性

Belongs to the small GTPase superfamily. Rab family.
翻訳後修飾

Phosphorylated by CDK1 kinase during mitosis.
Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.
細胞内局在

Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm, cytosol. Membrane. Melanosome. Alternates between membrane-associated and cytosolic forms.
Target information above from: UniProt accession P62820 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5861 Human
- Entrez Gene: 19324 Mouse
- Entrez Gene: 81754 Rat
- Omim: 179508 Human
- SwissProt: P62820 Human
- SwissProt: P62821 Mouse
- SwissProt: Q6NYB7 Rat
- Unigene: 310645 Human
- Unigene: 271944 Mouse
- Unigene: 106743 Rat
see all
別名
- GTP binding protein RAB 1A antibody
- mKIAA3012 antibody
- RAB 1 antibody
- Rab 1A antibody
- RAB1 antibody
- RAB1, member RAS oncogene family antibody
- Rab1A antibody
- RAB1A member RAS oncogene family antibody
- RAB1A_HUMAN antibody
- Ras related protein Rab 1A antibody
- Ras-associated protein RAB1 antibody
- Ras-related protein Rab-1A antibody
- YPT1 antibody
- YPT1 related protein antibody
- YPT1-related protein antibody
see all

Western blot - Anti-Rab1A antibody [EPR27169-83] (ab302545)

All lanes : Anti-Rab1A antibody [EPR27169-83] (ab302545) at 1/200 dilution

Lane 1 : Wild-type HAP1 lysate
Lane 2 : RAB1A knock-out HAP1 lysate
Lane 3 : RAB1B knock-out HAP1 lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

ab302545 was shown to react with RAB1A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB1A knockout cell line. Wild-type HAP1, RAB1A knockout and RAB1B knockoutcell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab302545 overnight at 4°C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Western blot - Anti-Rab1A antibody [EPR27169-83] (AB302545)

All lanes : Anti-Rab1A antibody [EPR27169-83] (ab302545) at 1/1000 dilution

Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Low expression: HCT116 (PMID: 30896866 ).
Western blot - Anti-Rab1A antibody [EPR27169-83] (AB302545)

All lanes : Anti-Rab1A antibody [EPR27169-83] (ab302545) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 4 : Human stomach tissue lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Rat brain tissue lysate
Lane 8 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Low expression: liver (PMID: 26308575).

Exposure time Lane: 1-4, 7-6: 126 seconds, Lane: 5-6: 15 seconds
Dot Blot - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Dot blot analysis of Rab1A using AB302545 at 1:1000 dilution (0.502 µg/mL) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lane 1: Human Ras-related protein Rab-1A immunogen peptide
Lane 2: Human Ras-related protein Rab-1B corresponding peptide

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Rab1A with ab302545 at 1/500 dilution (1.004 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in human stomach. The section was incubated with ab302545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Rab1A with ab302545 at 1/2000 dilution (0.251 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in mouse kidney (PMID:33730578). The section was incubated with ab302545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Rab1A with ab302545 at 1/2000 dilution (0.251 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in rat kidney (PMID:33730578). The section was incubated with ab302545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunocytochemistry/ Immunofluorescence - Anti-Rab1A antibody [EPR27169-83] (ab302545)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling Rab1A with AB302545 at 1/500 dilution (1.004 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preabsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in A549 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preabsorbed at 1/1000 dilution (2 µg/mL).
Immunocytochemistry/ Immunofluorescence - Anti-Rab1A antibody [EPR27169-83] (ab302545)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labeling Rab1A with AB302545 at 1/500 dilution (1.004 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preabsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in C2Cl2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preabsorbed at 1/1000 dilution (2 µg/mL).
Flow Cytometry (Intracellular) - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (human lung carcinoma epithelial cell) cells labeling Rab1A with AB302545 at 1/50 dilution (1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C2C12 (mouse myoblasts myoblast) cells labeling Rab1A with AB302545 at 1/50 dilution (1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Rab1A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell), whole cell lysate with AB302545 at 1/30 dilution. Western blot was performed on the immunoprecipitate using AB302545 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: A549 (human lung carcinoma epithelial cell), whole cell lysate 10 µg (Inset)

Lane 2: AB302545 IP in A549 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302545 in A549 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes

Observed MW (kDa): 22.
Immunoprecipitation - Anti-Rab1A antibody [EPR27169-83] (AB302545)

Rab1A was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate with AB302545 at 1/30 dilution. Western blot was performed on the immunoprecipitate using AB302545 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: C2C12 (mouse myoblasts myoblast), whole cell lysate 10 µg (Inset)

Lane 2: AB302545 IP in C2C12 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302545 in C2C12 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

Observed MW (kDa): 22.
Anti-Rab1A antibody [EPR27169-83] (AB302545)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab302545 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302545 は 2 報の論文で使用されています。

Li H et al. Prognosis value and positive association of Rab1A/IL4Rα aberrant expression in gastric cancer. Sci Rep 13:6964 (2023). PubMed: 37117331
Liu Y et al. Rab1A-Mediated Exosomal Sorting of miR-200c Enhances Breast Cancer Lung Metastasis. Breast Cancer (Dove Med Press) 15:403-419 (2023). PubMed: 37274058

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (2)

レビューと Q&A