AB228354

Anti-Rab1A 抗体

Anti-Rab1A antibody

JP Deleterious:医薬用外劇物

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(2 Publications)

Rabbit Polyclonal RAB1A antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB1A.

別名を表示する

RAB1, RAB1A, Ras-related protein Rab-1A, YPT1-related protein

4 Images

Supplier Data

Western blot - Anti-Rab1A antibody (AB228354)

All lanes:

Western blot - Anti-Rab1A antibody (ab228354) at 1/500 dilution

Lane 1:

U87-MG whole cell extract at 30 µg

Lane 2:

SK-N-SH whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-Rab1A antibody (AB228354)

All lanes:

Western blot - Anti-Rab1A antibody (ab228354) at 1/500 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-Rab1A antibody (AB228354)

Signal was developed with Trident ECL plus-Enhanced

All lanes:

Western blot - Anti-Rab1A antibody (ab228354) at 1/500 dilution

All lanes:

Mouse colon tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

true

Supplier Data

Western blot - Anti-Rab1A antibody (AB228354)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Rab1A antibody (ab228354) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

Rab1A transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 22 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human RAB1A. The exact immunogen used to generate this antibody is proprietary information.

P62820

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab1A also known as Ras-related protein Rab-1A is a small GTPase belonging to the Rab family. Rab1A has a role in regulating vesicle trafficking and it is involved in ER-to-Golgi transport. This protein with a molecular mass of approximately 23.5 kDa regulates intracellular traffic affecting vesicle budding motility and fusion. Rab1A is highly expressed in various tissues including the liver heart kidney and to lesser extents in others.

Biological function summary

The Rab1A protein influences intracellular transport processes and maintains cellular homeostasis. It forms part of multimeric protein complexes that facilitate its functions in the secretory pathway. The protein ensures the proper trafficking of cargo proteins from the endoplasmic reticulum to the Golgi apparatus playing a role in the early secretory pathway. This involvement is critical for maintaining the post-translational modification and processing of proteins.

Pathways

The mechanical function of Rab1A integrates into several cellular pathways including the secretory pathway and autophagy. Rab1A works with other proteins like Rab2 and Rab30 in the secretory pathway to coordinate the proper flow of proteins. Its coordination with proteins like LC3 during autophagy highlights its importance in cellular turnover and degradation processes linking Rab1A to essential biological functions and homeostasis.

Rab1A has a connection to certain cancers and neurodegenerative diseases. Aberrant Rab1A expression or mutations can lead to disruptions in cellular trafficking implicated in hepatocellular carcinoma. The protein's altered function could also influence the pathogenesis of Parkinson’s disease with proteins such as α-synuclein showing interaction in disease pathways. Understanding these relationships helps in elucidating Rab1A's role in disease mechanisms.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 20639577, PubMed : 20861236, PubMed : 21303926, PubMed : 22939626). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 20639577, PubMed : 20861236, PubMed : 21303926, PubMed : 22939626). RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion (PubMed : 21303926). Required to modulate the compacted morphology of the Golgi (PubMed : 26209634). Regulates the level of CASR present at the cell membrane (PubMed : 20861236). Plays a role in cell adhesion and cell migration, via its role in protein trafficking (PubMed : 20639577). Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria (PubMed : 22939626). Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport (By similarity).

See full target information RAB1A

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

NPJ microgravity 10:18 PubMed38365862

2024

Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanping Ren,Eun Hyun Ahn,Minjae Do,Devin B Mair,Amir Monemianesfahani,Peter H U Lee,Deok-Ho Kim

ASN neuro 13:17590914211049056 PubMed34806436

2021

Mechanism of Incisional Pain: Novel Finding on Long Noncoding RNA XIST/miR-340-5p/RAB1A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liao,Fan Zhang,Wenxiang Qing,Rili Yu,Zhonghua Hu

View all publications

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Abcam product promise

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Anti-Rab1A 抗体

Anti-Rab1A antibody

Western blot - Anti-Rab1A antibody (AB228354)

Western blot - Anti-Rab1A antibody (AB228354)

Western blot - Anti-Rab1A antibody (AB228354)

Western blot - Anti-Rab1A antibody (AB228354)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise