Anti-RAB11B 抗体 (ab3612)
Key features and details
- Rabbit polyclonal to RAB11B
- Suitable for: ICC, WB
- Reacts with: Dog, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RAB11B antibody
RAB11B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAB11B -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Dog, Human
交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Human RAB11B aa 1-100.
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ポジティブ・コントロール
- WB: canine MDCK, human PC3; ICC: HeLa
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3612の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
1/50 - 1/200.
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract.
Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
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特記事項 |
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ICC
1/50 - 1/200. |
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa. |
ターゲット情報
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機能
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
翻訳後修飾
Citrullinated by PADI4. -
細胞内局在
Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus. - Information by UniProt
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参照データベース
- Entrez Gene: 420063 Chicken
- Entrez Gene: 532723 Cow
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 100513782 Pig
- Entrez Gene: 79434 Rat
- Omim: 604198 Human
- SwissProt: Q3MHP2 Cow
see all -
別名
- GTP-binding protein ypt3 antibody
- H YPT3 antibody
- RAB11B antibody
see all
画像
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Immunocytochemical analysis of 4% paraformaldeehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells treated/untreated with actinomycin D. Rab11B is stained with ab3612 at a 1/100 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a 1/500 dilution (green). Nuclear counterstaining is using Hoechst 33342 (blue).
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Anti-RAB11B antibody (ab3612) at 1/500 dilution + MDCK (canine kidney cell line) whole cell lysate at 20 µg
Predicted band size: 24 kDa -
ab3612 used on PC3 cell extract.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab3612 は 36 報の論文で使用されています。
- Li YY et al. Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation. Cell Mol Gastroenterol Hepatol 15:533-558 (2023). PubMed: 36270602
- Chen XN et al. Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport. J Virol 97:e0021023 (2023). PubMed: 36975780
- Solinger JA et al. FERARI and cargo adaptors coordinate cargo flow through sorting endosomes. Nat Commun 13:4620 (2022). PubMed: 35941155
- Oh Y et al. SPIN90 Deficiency Ameliorates Amyloid β Accumulation by Regulating APP Trafficking in AD Model Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36142484
- Barbet G et al. TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming. Nat Immunol 22:497-509 (2021). PubMed: 33790474