AB230261

Anti-RAB10 (phospho T73) 抗体 [MJF-R21]

Anti-RAB10 (phospho T73) antibody [MJF-R21]

20ul selling size
RabMAb
Recombinant
KO Validated
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(90 Publications)

Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) is a rabbit monoclonal antibody detecting RAB10 in Western Blot, Dot Blot. Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

別名を表示する

Ras-related protein Rab-10, RAB10

5 Images

PubMed

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% BSA/TBST.

The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID : 29127256).

Scanned with Licor Odyssey CLx.

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1:

Wild-type A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Wild-type A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 3:

Rab8A knock-out A549 whole cell lysate at 20 µg

Lane 4:

Rab8A knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 5:

Rab10 knock-out A549 whole cell lysate at 20 µg

Lane 6:

Rab10 knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

HRP-labeled secondary antibody at 1/2500 dilution

Predicted band size: 22 kDa

Observed band size: 23 kDa

false

Supplier Data

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Blocking/Dilution buffer : 5% NFDM/TBST.

The LRRK2 pathogenic mutation R1441C increases LRRK2 activity and markedly elevates Rab10 phosphorylation in MEF (mouse embryonic fibroblasts).

The expression pattern is consistent with the literature (PMID : 29127256).

The cell lysates were kindly provided by our collaborator, Dr. Dario Alessi.

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1:

Wild-type MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

Wild-type MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 3:

LRRK2 [R1441C] knock-in MEF whole cell lysate at 20 µg

Lane 4:

LRRK2 [R1441C] knock-in MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa

Observed band size: 23 kDa

true

Exposure time: 3min

PubMed

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% BSA/TBST.

The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins.

The images were kindly provided by our collaborator Dr. Dario Alessi, and have been published (PMID : 29127256).

Scanned with Licor Odyssey CLx.

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole at 0.1 µg

Lane 2:

HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

Lane 3:

HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab10 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

Lane 4:

HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

Lane 5:

HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab43 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

Secondary

All lanes:

IRDye 800CW secondary antibody at 1/25000 dilution

Predicted band size: 22 kDa

Observed band size: 23 kDa

false

Lab

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Observed MW 28 kDa

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with RAB10 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Dot blot analysis of Rab10 (phospho T73) labeled with ab230261 at 1/1000 dilution.

Lane 1 : Rab10 (phospho T73) peptide;

Lane 2 : Rab10 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 32 seconds.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

MJF-R21

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human, Mouse samples.

What is the molecular weight of RAB10?
Anti-RAB10 (phospho T73) [MJF-R21] (ab230261) specifically detects a band for RAB10 (UniProt: P61026) at a molecular weight of 23kDa.

Trusted by the scientific community
Anti-RAB10 (phospho T73) [MJF-R21] (ab230261) was first used in a scientific publication in 2018 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) has been confirmed by Western blot testing in RAB10 Knockout MEF cells.

Other related products
We have a range of other formats of antibody clone [MJF-R21] also available for your convenience: ab230261, Carrier free - ab231707, ab315174, Carrier free - ab315175

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.

Biological function summary

RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.

Pathways

RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.

RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21248164). RAB10 is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed : 21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, RAB10 regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed : 16641372). In neurons, involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, regulates transport from the Golgi to the basolateral membrane (PubMed : 16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed : 23263280). Together with LRRK2, RAB8A, and RILPL1, regulates ciliogenesis (PubMed : 30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed : 30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed : 32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed : 30209220).. (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.

See full target information RAB10 phospho T73

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文献 (90)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed41031878

2025

Targeting specific kinase substrates rescues increased colitis severity induced by the Crohn's disease-linked LRRK2-N2081D variant.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Xingjian Li,Xianting Li,Xiaoting Zhou,Yuanxi Zhang,Duc Tung Vu,Marc Oeller,Ozge Karayel,Quyen Q Hoang,Meltem Ece Kars,Nitika Kamath,Minghui Wang,Leonid Tarassishin,Matthias Mann,Inga Peter,Zhenyu Yue

Cells 14: PubMed40801573

2025

The Interaction of pT73-Rab10 with Myosin Va, but Not Myosin Vb, Is Regulated Though a Site in the Globular Tail Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Lynne A Lapierre,Elizabeth H Manning,Kyra S Thomas,Catherine Caldwell,James R Goldenring

Cell reports 44:116031 PubMed40690364

2025

PPM1M, an LRRK2-counteracting, phosphoRab12-preferring phosphatase with a potential link to Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Y Chiang,Neringa Pratuseviciute,Yu-En Lin,Ayan Adhikari,Wondwossen M Yeshaw,Chloe Flitton,Pemba L Sherpa,Francesca Tonelli,Irena Rektorova,Timothy Lynch,Joanna Siuda,Monika Rudzińska-Bar,Oleksandr Pulyk,Peter Bauer,Christian Beetz,Dennis W Dickson,Owen A Ross,Zbigniew K Wszolek,Zih-Hua Fang,Christine Klein,Alexander Zimprich,Dario R Alessi,Esther M Sammler,Suzanne R Pfeffer

EMBO reports 26:3327-3356 PubMed40394349

2025

Inflammation and IL-4 regulate Parkinson's and Crohn's disease associated kinase LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Dikovskaya,Rebecca Pemberton,Matthew Taylor,Anna Tasegian,Purbasha Bhattacharya,Karolina Zeneviciute,Esther M Sammler,Andrew J M Howden,Dario R Alessi,Mahima Swamy

International journal of molecular sciences 26: PubMed40332013

2025

Environmental Factors Exacerbate Parkinsonian Phenotypes in an Asian-Specific Knock-In LRRK2 Risk Variant in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zoë Bichler,Sarivin Vanan,Zhiwei Zhang,Qianying Sally Dong,Jolene Wei Ling Lee,Chengwu Zhang,Liting Hang,Mei Jiang,Parasuraman Padmanabhan,Wuan Ting Saw,Zhidong Zhou,Balázs Gulyás,Kah Leong Lim,Li Zeng,Eng King Tan

NPJ Parkinson's disease 11:61 PubMed40155632

2025

The homozygous LRRK2.p.N1437D point mutation mouse is a novel model of parkinsonism.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Hua Gan,Yi-Min Sun,Xin-Yue Zhou,Zhi-Yuan Qi,Feng-Tao Liu,Yi-Lin Tang,Wen-Bo Yu,Bao-Guo Xiao,Jian Wang,Jian-Jun Wu

Nature communications 16:2329 PubMed40057496

2025

Salmonella exploits LRRK2-dependent plasma membrane dynamics to invade host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxian Zhu,Andrew M Sydor,Bing-Ru Yan,Ren Li,Michal T Boniecki,Carina Lyons,Miroslaw Cygler,Aleixo M Muise,Michelle E Maxson,Sergio Grinstein,Brian Raught,John H Brumell

NPJ Parkinson's disease 11:34 PubMed39988587

2025

Clinical and functional evidence for the pathogenicity of the LRRK2 p.Arg1067Gln variant.

Applications

Unspecified application

Species

Unspecified reactive species

Shen-Yang Lim,Tzi Shin Toh,Jia Wei Hor,Jia Lun Lim,Lei Cheng Lit,Azlina Ahmad-Annuar,Yi Wen Tay,Jia Nee Foo,Ebonne Yulin Ng,Kalai Arasu Muthusamy,Norlinah Mohamed Ibrahim,Khairul Azmi Ibrahim,Louis Chew Seng Tan,Jannah Zulkefli,Anis Nadhirah Khairul Anuar,Kirsten Black,Pawel Lis,Fei Xie,Zhidong Cen,Kai Shi Lim,Katja Lohmann,Shalini Padmanabhan,Dario R Alessi,Wei Luo,Eng King Tan,Esther Sammler,Ai Huey Tan

Chemical science 16:3430-3439 PubMed39877816

2025

Functionally active modulators targeting the LRRK2 WD40 repeat domain identified by FRASE-bot in CACHE Challenge #1.

Applications

Unspecified application

Species

Unspecified reactive species

Akhila Mettu,Marta Glavatskikh,Xiaowen Wang,Antonio Jesús Lara Ordóñez,Fengling Li,Irene Chau,Suzanne Ackloo,Cheryl Arrowsmith,Albina Bolotokova,Pegah Ghiabi,Elisa Gibson,Levon Halabelian,Scott Houliston,Rachel J Harding,Ashley Hutchinson,Peter Loppnau,Sumera Perveen,Almagul Seitova,Hong Zeng,Matthieu Schapira,Jean-Marc Taymans,Dmitri Kireev

iScience 27:110980 PubMed39635134

2024

Rebalance of mitophagy by inhibiting LRRK2 improves colon alterations in an MPTP model.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Filippone,Deborah Mannino,Laura Cucinotta,Fabrizio Calapai,Lelio Crupi,Irene Paterniti,Emanuela Esposito

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB10 (phospho T73) 抗体 [MJF-R21]

Anti-RAB10 (phospho T73) antibody [MJF-R21]

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (90)

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Abcam product promise