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リコンビナントRabMAb

Anti-Quinone oxidoreductase 抗体 [EPR10843(B)] (ab154842)

Submit a review Submit a question References (1)
Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Quinone oxidoreductase antibody [EPR10843(B)]
    Quinone oxidoreductase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal liver lysate; HepG2 and K562 cell lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154842の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.

  • 組織特異性

    Only very low amounts in the lens.

  • 配列類似性

    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Crystallin zeta (quinone reductase) antibody
      • Crystallin zeta antibody
      • Cryz antibody
      • DKFZp779E0834 antibody
      • FLJ41475 antibody
      • NADPH:quinone reductase antibody
      • QOR_HUMAN antibody
      • Quinone oxidoreductase antibody
      • Zeta crystallin antibody
      • Zeta-crystallin antibody
      see all

    画像

    • Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      All lanes : Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunofluorescence analysis of HepG2 cells, labeling Quinone oxidoreductase using ab154842 at a 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

    参考文献 (1)

    ab154842 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154842 は 1 報の論文で使用されています。

    • Garzón I  et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol 8:681 (2020). PubMed: 32671048

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