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RabMAb

Anti-Pyroglutamate beta Amyloid 抗体 [IBR-10-2-3] - BSA and Azide free (ab264035)

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Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
  • Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
  • Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
  • Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)

Key features and details

  • Rabbit monoclonal [IBR-10-2-3] to Pyroglutamate beta Amyloid - BSA and Azide free
  • Suitable for: WB, Dot blot, Indirect ELISA
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free
    Pyroglutamate beta Amyloid 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [IBR-10-2-3] to Pyroglutamate beta Amyloid - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blot, Indirect ELISAmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: beta Amyloid pE3-42 (aa674-713) peptide, beta Amyloid 1-42 (aa672-713) peptide Indirect ELISA: Human Pyroglutamate amyloid beta (pE3-42A), Human Amyloid beta peptide (Ab42) Dot blot: beta Amyloid pE3-42 (aa674-713) peptide, beta Amyloid 1-42 (aa672-713) peptide

  • 特記事項

    ab244035 is a carrier free version of ab264034. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    IBR-10-2-3

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab264035の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 8 kDa.
Dot blot
1/2000.
Indirect ELISA
Use at an assay dependent concentration.

Use at 62.5ng/ml
特記事項
WB
1/1000. Detects a band of approximately 8 kDa.
Dot blot
1/2000.
Indirect ELISA
Use at an assay dependent concentration.

Use at 62.5ng/ml
追加情報
Is unsuitable for IHC-P.

画像

  • Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
    Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
    All lanes : Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034) at 1/1000 dilution

    Lane 1 : beta Amyloid pE3-42 (aa674-713) peptide 100ng
    Lane 2 : beta Amyloid 1-42 (aa672-713) peptide 100ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution

    Exposure time: 3 seconds


    This data was developed using ab264034, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

  • Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
    Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)

    This data was developed using ab264034, the same antibody clone in a different buffer formulation. 

    Indirect ELISA antibody dose-response curve using ab264034 with antigen at 1000 ng/ml

  • Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
    Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)

    This data was developed using ab264034, the same antibody clone in a different buffer formulation. 

    Dot blot analysis of pyroglutamate beta amyloid  using ab264034 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

    Lane 1: beta Amyloid pE3-42 (aa674-713) peptide

    Lane 2: beta Amyloid 1-42 (aa672-713) peptide

    Exposure time: 3 minutes. 

  • Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)
    Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] - BSA and Azide free (ab264035)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab264035 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab264035 は論文での使用が確認できていません。

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