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RabMAb

Anti-Pyroglutamate beta Amyloid 抗体 [IBR-10-2-3] (ab264034)

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Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
  • Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
  • Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
  • Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)

Key features and details

  • Rabbit monoclonal [IBR-10-2-3] to Pyroglutamate beta Amyloid
  • Suitable for: WB, Dot blot, Indirect ELISA
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3]
    Pyroglutamate beta Amyloid 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [IBR-10-2-3] to Pyroglutamate beta Amyloid

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blot, Indirect ELISAmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: beta Amyloid pE3-42 (aa674-713) peptide, beta Amyloid 1-42 (aa672-713) peptide Indirect ELISA: Human Pyroglutamate amyloid beta (pE3-42A), Human Amyloid beta peptide (Ab42) Dot blot: beta Amyloid pE3-42 (aa674-713) peptide, beta Amyloid 1-42 (aa672-713) peptide

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    IBR-10-2-3

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab264034の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000.
Dot blot
1/2000.
Indirect ELISA
Use a concentration of 0.0625 µg/ml.
特記事項
WB
1/1000.
Dot blot
1/2000.
Indirect ELISA
Use a concentration of 0.0625 µg/ml.
追加情報
Is unsuitable for IHC-P.

画像

  • Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
    Western blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
    All lanes : Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034) at 1/1000 dilution

    Lane 1 : beta Amyloid pE3-42 (aa674-713) peptide 100ng
    Lane 2 : beta Amyloid 1-42 (aa672-713) peptide 100ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution

    Exposure time: 3 seconds


    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

  • Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
    Indirect ELISA - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)

    Indirect ELISA antibody dose-response curve using ab264034 with antigen at 1000 ng/ml

  • Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
    Dot Blot - Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)

    Dot blot analysis of pyroglutamate beta amyloid  using ab264034 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

    Lane 1: beta Amyloid pE3-42 (aa674-713) peptide

    Lane 2: beta Amyloid 1-42 (aa672-713) peptide

    Exposure time: 3 minutes. 

  • Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)
    Anti-Pyroglutamate beta Amyloid antibody [IBR-10-2-3] (ab264034)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab264034 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab264034 は論文での使用が確認できていません。

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