Anti-PYK2 抗体 [E354] (ab32448)
![Western blot - Anti-PYK2 antibody [E354] (ab32448)](https://www.abcam.co.jp/ps/products/32/ab32448/Images/ab32448-281782-anti-pyk2-antibody-e354-western-blot.jpg "Western blot - Anti-PYK2 antibody [E354] (ab32448)")
Key features and details
- Rabbit monoclonal [E354] to PYK2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-PYK2 antibody [E354]
PYK2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E354] to PYK2 -
由来種
Rabbit -
特異性
The antibody does not cross-react with other Fak family members. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat whole cell lysate (ab7899).
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
E354 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
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| 特記事項 |
|---|
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WB
1/500. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
ターゲット情報
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機能
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption. -
組織特異性
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. -
細胞内局在
Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase. - Information by UniProt
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参照データベース
- Entrez Gene: 2185 Human
- Entrez Gene: 19229 Mouse
- Entrez Gene: 50646 Rat
- Omim: 601212 Human
- SwissProt: Q14289 Human
- SwissProt: Q9QVP9 Mouse
- SwissProt: P70600 Rat
- Unigene: 491322 Human
see all -
別名
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
see all
画像
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Western blot - Anti-PYK2 antibody [E354] (ab32448)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PYK2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hu brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32448 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32448 was shown to specifically react with PYK2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PYK2 knockout samples were used. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE. ab32448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PYK2 antibody [E354] (ab32448)](https://www.abcam.co.jp/ps/products/32/ab32448/Images/ab32448-44886-anti-pyk2-antibody-e354-western-blot.gif)
Western blot - Anti-PYK2 antibody [E354] (ab32448)Anti-PYK2 antibody [E354] (ab32448) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 116 kDa
Observed band size: 116 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab32448 は 9 報の論文で使用されています。
- Yang CM et al. Rhamnetin Prevents Bradykinin-Induced Expression of Matrix Metalloproteinase-9 in Rat Brain Astrocytes by Suppressing Protein Kinase-Dependent AP-1 Activation. Biomedicines 11:N/A (2023). PubMed: 38137419
- Lin YJ et al. Reactive Oxygen Species-Dependent Activation of EGFR/Akt/p38 Mitogen-Activated Protein Kinase and JNK1/2/FoxO1 and AP-1 Pathways in Human Pulmonary Alveolar Epithelial Cells Leads to Up-Regulation of COX-2/PGE2 Induced by Silica Nanoparticles. Biomedicines 11:N/A (2023). PubMed: 37893002
- Yang CC et al. HO-1 Upregulation by Kaempferol via ROS-Dependent Nrf2-ARE Cascade Attenuates Lipopolysaccharide-Mediated Intercellular Cell Adhesion Molecule-1 Expression in Human Pulmonary Alveolar Epithelial Cells. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453467
- Yang CC et al. Galangin Inhibits LPS-Induced MMP-9 Expression via Suppressing Protein Kinase-Dependent AP-1 and FoxO1 Activation in Rat Brain Astrocytes. J Inflamm Res 13:945-960 (2020). PubMed: 33244253
- Yang CM et al. Mevastatin-Induced AP-1-Dependent HO-1 Expression Suppresses Vascular Cell Adhesion Molecule-1 Expression and Monocyte Adhesion on Human Pulmonary Alveolar Epithelial Cells Challenged with TNF-a. Biomolecules 10:N/A (2020). PubMed: 32121588
- Yang CC et al. RTA 408 Inhibits Interleukin-1ß-Induced MMP-9 Expression via Suppressing Protein Kinase-Dependent NF-?B and AP-1 Activation in Rat Brain Astrocytes. Int J Mol Sci 20:N/A (2019). PubMed: 31185608
- Yang CC et al. Lipopolysaccharide-Induced Matrix Metalloproteinase-9 Expression Associated with Cell Migration in Rat Brain Astrocytes. Int J Mol Sci 21:N/A (2019). PubMed: 31905967
- Cho RL et al. Haem oxygenase-1 up-regulation by rosiglitazone via ROS-dependent Nrf2-antioxidant response elements axis or PPAR? attenuates LPS-mediated lung inflammation. Br J Pharmacol 175:3928-3946 (2018). PubMed: 30088830
- Milatovic D et al. Mefloquine neurotoxicity is mediated by non-receptor tyrosine kinase. Neurotoxicology 32:578-85 (2011). PubMed: 21241737