Anti-PYGO2 抗体 (ab99274)
Key features and details
- Rabbit polyclonal to PYGO2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PYGO2 antibody
PYGO2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PYGO2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human PYGO2 aa 1-50 (N terminal).
Database link: NP_612157.1 -
ポジティブ・コントロール
- HeLa, 293T and Mouse NIH3T3 whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99274の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in signal transduction through the Wnt pathway. -
配列類似性
Contains 1 PHD-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 90780 Human
- Entrez Gene: 68911 Mouse
- Entrez Gene: 295251 Rat
- Omim: 606903 Human
- SwissProt: Q9BRQ0 Human
- Unigene: 533597 Human
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別名
- 1190004M21Rik antibody
- FLJ33226 antibody
- PP7910 antibody
see all
画像
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All lanes : Anti-PYGO2 antibody (ab99274) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PYGO2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
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ab99274, at 0.4 µg/ml, detecting PYGO2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
Lane 1; IP using ab99274
Lane 2; IP using a rabbit anti-PYGO2 antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99274 は論文での使用が確認できていません。