AB231963

Anti-PYGM 抗体

Name: Anti-PYGM 抗体 (ab231963) | Abcam
Brand: Abcam Limited
SKU: AB231963
Price: 74000 JPY
Availability: InStock

Anti-PYGM antibody

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal PYGM antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pygm aa 1-200.

別名を表示する

Myophosphorylase, PYGM

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGM antibody (AB231963)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGM antibody (AB231963)

Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for PYGM using ab231963 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

Recombinant fragment (His-tag) corresponding to Mouse PYGM aa 11-187, 22kDa.

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Recombinant mouse PYGM protein

Predicted band size: 97 kDa

false

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Mouse skeletal muscle lysate

Predicted band size: 111 kDa,77 kDa,97 kDa

false

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Mouse liver lysate

Predicted band size: 97 kDa

false

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Rat skeletal muscle lysate

Predicted band size: 97 kDa

false

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 97 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Mouse Pygm aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9WUB3

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

バッファー組成

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PYGM also known as muscle glycogen phosphorylase or myophosphorylase is an enzyme with a molecular mass of around 97 kDa. This enzyme plays a mechanical role in glycogen metabolism by catalyzing the breakdown of glycogen to glucose-1-phosphate. PYGM is particularly expressed in skeletal muscles where it aids in mobilizing stored energy during periods of increased demand such as exercise.

Biological function summary

The function of PYGM is critical in energy homeostasis within muscle tissue. This enzyme forms part of the glycogen phosphorylase complex which facilitates rapid energy release by converting glycogen into glucose-1-phosphate. This release is important for maintaining adequate levels of glucose for muscle contraction during activity or stress. Its activity is modulated by various factors including allosteric changes and phosphorylation processes ensuring that glucose metabolism aligns with the cellular energy requirements.

Pathways

PYGM integrates mainly into the glycolytic pathway where it provides glucose-1-phosphate as a substrate for glycolysis enhancing ATP production. The enzyme also plays an important role in the glycogenolysis pathway orchestrating energy release from glycogen stores. PYGM functions in connection with related proteins such as glycogen debranching enzyme and phosphoglucomutase working in concert to maintain energy balance and inform the overall metabolic response during physical exertion.

Defects in PYGM relate to McArdle disease a metabolic muscle disorder characterized by exercise intolerance and myoglobinuria. Deficiencies in PYGM impair glycogen breakdown in muscles leading to reduced availability of glucose for energy and resulting in muscle fatigue and pain. This disorder often links with metabolic consequences affecting enzymes involved in glucose handling such as muscle adenylate kinase highlighting the interconnected nature of metabolic pathways and disease pathology.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.

See full target information PYGM

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2821 PubMed40121188

2025

Smoking aggravates neovascular age-related macular degeneration via Sema4D-PlexinB1 axis-mediated activation of pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kai He,Xue Dong,Tianjing Yang,Ziqi Li,Yuming Liu,Jing He,Meng Wu,Selena Wei-Zhang,Parhat Kaysar,Bohao Cui,Xueming Yao,Li Zhang,Wei Zhou,Heping Xu,Jun Wei,Qiang Liu,Junhao Hu,Xiaohong Wang,Hua Yan

CNS neuroscience & therapeutics 30:e70065 PubMed39350328

2024

Comprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Xin-Yi Liu,Qi-Fang He,Fu-Ze Zheng,Long Chen,Ying Zheng,Ming-Hui Zeng,Yu-Hua Lin,Xin Lin,Hai-Zhu Chen,Min-Ting Lin,Ning Wang,Zhi-Qiang Wang,Feng Lin

Reproduction (Cambridge, England) 168: PubMed39226129

2024

Membrane progesterone receptors mediate progesterone-stimulated glycogenolysis in the bovine uterine epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Malia D Berg,Matthew Dean

PloS one 19:e0295964 PubMed38289946

2024

A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Eun Kwak,Amy Zheng,Edward B Arias,Haiyan Wang,Xiufang Pan,Yongping Yue,Dongsheng Duan,Gregory D Cartee

Molecular reproduction and development 89:431-440 PubMed35842832

2022

Endometrial glycogen metabolism during early pregnancy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ziting Chen,Kassandra Sandoval,Matthew Dean

Frontiers in microbiology 11:557039 PubMed33329421

2020

Glycogen Phosphorylase: A Drug Target of Amino Alcohols in , Predicted by a Computer-Aided Method.

Applications

Unspecified application

Species

Unspecified reactive species

Congshan Liu,Jianhai Yin,Wei Hu,Haobing Zhang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYGM 抗体

Anti-PYGM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGM antibody (AB231963)

Western blot - Anti-PYGM antibody (AB231963)

Western blot - Anti-PYGM antibody (AB231963)

Western blot - Anti-PYGM antibody (AB231963)

Western blot - Anti-PYGM antibody (AB231963)

Western blot - Anti-PYGM antibody (AB231963)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise