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リコンビナントRabMAb

Anti-PUS7 抗体 [EPR25172-31] - BSA and Azide free (ab289865)

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Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Flow Cytometry (Intracellular) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
  • Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25172-31] to PUS7 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free
    PUS7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25172-31] to PUS7 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    This antibody does not react with Human species for IHC, ICC and FC application.

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, PC-12, 293T, NIH/3T3 and HCT116 whole cells lysates; Mouse liver, tissue lysate; Rat pancreas and liver tissues lysates. IHC-P: Rat colon tissue; Mouse colon tissue. ICC/IF: NIH/3T3 cells. Flow cyt: NIH/3T3 cells.

  • 特記事項

    ab289865 is the carrier-free version of ab289857.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289865の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

画像

  • Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    All lanes : Anti-PUS7 antibody [EPR25172-31] (ab289857) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 5 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75, 34 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer. 

    Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.
    We observe an unknown band at around 37kDa. 

     

  • Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Western blot - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    All lanes : Anti-PUS7 antibody [EPR25172-31] (ab289857) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Rat pancreas tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer. 

    Exposure times: 

    Lane 1: 81 seconds
    Lanes 2-3: 180 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue lysate labeling PUS7 at 1/1000 (1.374μg/ml), followed by LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on mouse colon. The section was incubated with ab289857 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.  Counter stained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    econdary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded rat colon tissue lysate labeling PUS7 at 1/1000 (1.374μg/ml), followed by LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat colon. The section was incubated with ab289857 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling PUS7 with ab289857 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterstain is DAPI (Blue). Tubulin is labeled using ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution. 

  • Flow Cytometry (Intracellular) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Flow Cytometry (Intracellular) - Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

    This data was developed using ab289857, the same antibody clone in a different buffer formulation. 

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilised NIH/3T3 (Mouse embryonic fibroblast cell line) cells labelling PUS7 with ab289857 at 1/500 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody. 

  • Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)
    Anti-PUS7 antibody [EPR25172-31] - BSA and Azide free (ab289865)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289865 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289865 は論文での使用が確認できていません。

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